Studies on biomass and lipid production of seven diatom species with special emphasis on lipid composition of Nitzschia palea (Bacillariophyceae) as reliable biodiesel feedstock

Abdel-Hamid, Mohammed I.; El-Refaay, Dina A.; Abdel-Mogib, Μ.; Azab, Yehia A.

doi:10.1127/1864-1318/2013/0069

Cited by 14 publications

(11 citation statements)

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“…Nanomaterials with special physical and chemical properties, such as small size, surface area, adsorption, and catalysis have been shown to have a significant effect on plant growth (Li et al ), but their effect on algal cells has been rarely reported. Suman et al () showed that a nanosilica‐based micronutrient mixture Nualgi promoted growth in Cylindrotheca sp., and similar results were also observed in the freshwater diatom Nitzschia palea (Kützing) W.Smith (Abdel‐Hamid et al ). In this study, we used only nanosilica, which resulted in reduced cell density in Navicula sp.…”

Section: Discussionsupporting

confidence: 82%

Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates

Marella

Tao

et al. 2017

Phycological Research

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Diatoms are a group of highly abundant and diverse aquatic algae species. They contain high lipid content along with many bioactive compounds that can be exploited for biotechnological applications. Despite these attractive attributes, diatoms are underrepresented in production projects due to difficulties in their cultivation. To optimize the growth of three freshwater diatom isolates, Cyclotella sp., Synedra sp. and Navicula sp., an orthogonal assay on N, P, Si and Fe, as well as temperature and pH, was designed using traditional single-factor tests. We also studied the effect of using nanosilica as an alternate Si source on growth and found that the diatom isolates studied achieved their highest growth rates under different combinations of nutrient and environmental conditions. Silica had the greatest influence on growth, followed by phosphate and iron. The optimized growth conditions for Synedra sp. were N: 30 mg L −1 , P: 3 mg L −1 , Si: 14.8 mg L −1 , Fe: 0.448 mg L −1 , temperature 25 C and pH 8. For Navicula sp.: N: 20 mg L −1 , P: 2.5 mg L −1 , Si: 19.7 mg L −1 , Fe: 0.112 mg L −1 , temperature 30 C and pH 7.5-8. For Cyclotella sp.: N: 20 mg L −1 , P: 2.5 mg L −1 , Si: 19.7 mg L −1 , Fe: 0.448 mg L −1 , temperature 30 C and pH 7.5-8. Nano silica negatively affected growth in Navicula sp. and Cyclotella sp., but no such effect was observed in Synedra sp. Fatty acid profiling showed C16:0, C16:1(n − 7), C18:0 and C20:5(n − 3) as major fatty acids, with no significant differences in fatty acid methyl ester profiles between traditional and modified media. This work gives us a new insight into the growth requirements of freshwater diatom species, which are less studied than marine species.

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Section: Discussionsupporting

confidence: 82%

Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates

Marella

Tao

et al. 2017

Phycological Research

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“…Periodic variations in biomass dry weight and lipid production, however, were irregular with no distinct seasonal patterns. Similar results were reported for algal growth under natural environmental conditions ( Lebeau and Robert, 2003 , Lombardi and Wangersky, 1995 , Hu et al, 2008 , Thomas et al, 1983 , Van Gerpen et al, 2004 , Felizardo et al, 2006 , Demirbas, 2009 , Chisti, 2007 , Aoyagi and Neogene, 1992 , Holba et al, 1998 , Hu et al, 2006 , Ramachandra et al, 2009 , Abdel-Hamid et al, 2013 , Starr, 1978 , Cox, 1962 , Horowitz, 1975 , D, 2638 , D, 1310 , Christie and Christie, 1993 , Vicente et al, 2004 , Haas et al, 2009 , Chongkhong et al, 2007 , Goff et al, 2004 , Molnar-Perl and Pinter-Szakacs, 1986 , Sharma and Singh, 2009 , Griffiths and Harrison, 2009 , Sheehan et al, 1998 , Basha and Jebaraj, 2009 , Knothe, 2005 , Sadasivam et al, 1996 , Matsumoto et al, 2017 )and for lipid production by outdoor cultures of microalgae ( Starr, 1978 , Cox, 1962 , Horowitz, 1975 , D, 2638 , D, 1310 , Christie and Christie, 1993 , Vicente et al, 2004 , Haas et al, 2009 , Chongkhong et al, 2007 , Goff et al, 2004 , Molnar-Perl and Pinter-Szakacs, 1986 , Sharma and Singh, 2009 , Griffiths and Harrison, 2009 , Sheehan et al, 1998 , Basha and Jebaraj, 2009 , Knothe, 2005 , Sadasivam et al, 1996 , Matsumoto et al, 2017 ).…”

Section: Discussionsupporting

confidence: 81%

“…A previous study carried out by authors ( Abdel-Hamid et al, 2013 ) indicated that under favorable laboratory growth conditions, the pinnate diatom Nitzschia palea produced relatively higher quantities of biomass and lipids than other tested diatoms. In addition, gas chromatography/mass spectroscopy (GC/MS) analysis indicated the suitability of the structure and composition of N. palea derived fatty acids as potential feedstock for biodiesel.…”

Section: Introductionmentioning

confidence: 85%

Long-term monitoring of the biomass and production of lipids by Nitzschia palea for biodiesel production

Touliabah

Abdelhamid

Al-Mutairi

2020

Saudi Journal of Biological Sciences

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Pennate diatom Nitzschia palea can be cultured in outdoor vertical-bed photobioreactors to produce biodiesel. To assess the production of biomass and lipids, non-axenic cultures of Nitzschia palea were grown outdoors, and the growth of these cultures was measured biweekly. During the annual cycle of algal culture, the culture temperature ranged from 17.3 °C to 33.5 °C, the dry weight biomass ranged from 0.11 g l −1 to 0.25 g l −1 , light energy] ranged from 1.94 Wm −2 to 3.9 Wm −2 and intracellular lipid content ranged from 7.1% to 11.4% of biomass weight after drying at 60 °C. Gas chromatography/mass spectroscopy (GC/MS) analysis of n-hexane extracts showed that the intracellular lipids were primarily C14:0 myristic acid (9.01%), C15:0 pentadecyclic acid (8.26%) and two types of C16:0, palmitic acid (41.13%) and palmitoleic acid (29.25%). Gel permeation analysis showed that carboxylic acids comprised 28.9% of lipids, 16.3% of monoglycerides, 27.3% of diglycerides and 24.3% of triglycerides. Alcoholysis of lipids resulted in the conversion of about 93.9% of fatty acids to equivalent fatty acid methyl esters (FAME) or biodiesel, which, on basis of wt%, consisted primarily of C15:0 methyl myristate (8.3%), C16:0 methyl pentadecanoate] (7.2%), C17:1methyl palmitoleate (28.7%) and methyl palimtate](39.8%).

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“…Nitzschia palea was grown in different culture media with different nutrients and tested as a model diatom to reveal its suitability as biodiesel raw material. The results of these and similar studies revealed that more intensive and comprehensive studies are needed for production of biodiesel from N. palea (Abdel-Hamid et al 2013). In recent years, researchers have investigated the ability of Bacillariophyceae members to produce biologically active compounds and they have reported that they might be appropriate for antibiotic production.…”

Section: Introductionmentioning

confidence: 92%

“…Tentative assignment of bands found in the FTIR spectra of N. palea1 Cells, which are adapted to the new medium, start to grow and the culture reach a maximum concentration in 12-18 hours. This phase is known as the exponential phase.…”

mentioning

confidence: 99%

DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY

Duygu

2019

Trakya University Journal of Natural Sciences

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This study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species Nitzschia palea (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. The study material was isolated from a fresh water sample collected from Ankara, Turkey. The diatoms were cultured in Allen medium for 168 hours and the growth dynamics were determined by cell density and dry weight analyses. Specific growth rate, duplication time of the culture and biochemical compositions were also investigated. Molecular characterization of the N. palea strain was performed by applying Fourier Transform Infrared Spectroscopy. The cell density and the dry biomass of the culture at the end of the 168 hours incubation period was determined as 2.0x10 6 ±1.0x10 5 cells/mL and 0.212±0.041 g L-1 , respectively. The algal specific growth rate was found as 0.010 h-1 at 96-h and the doubling time was calculated as 68 h-1. The protein content was measured as 41.21%, carbohydrate content as 21.74%, lipid content as 16.84% and ash content as 19.88%. These results indicated that N. palea may be used in different fields of industries, especially in biodiesel production.

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Studies on biomass and lipid production of seven diatom species with special emphasis on lipid composition of Nitzschia palea (Bacillariophyceae) as reliable biodiesel feedstock

Cited by 14 publications

References 0 publications

Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates

Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates

Long-term monitoring of the biomass and production of lipids by Nitzschia palea for biodiesel production

DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY

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