Studies on Blood Coagulation: A Proteolytic Enzyme Prepared From Calcium and Platelet Free Normal Human Blood Plasma 1

Tagnon, Henri; Davidson, Charles S.; Taylor, F. J. R.

doi:10.1172/jci101329

Cited by 47 publications

(24 citation statements)

References 14 publications

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“…Pohle and Taylor (1937) first demonstrated that intramuscular use of this globulin substance was effective in correcting the clotting time in patients with haemophilia, but also demonstrated the refractory state induced by subsequent injections. The Thorndike also participated in the beginnings of the modern understanding of disseminated intravascular coagulation when Lozner and Taylor (1942) reported the effects of artificial surfaces on the initiation of coagulation and Tagnon et al (1942) studied the fibrinolytic activity of plasma. Lymphoma Henry Jackson Jr was the major contributor to morphological research in haematology at the Thorndike.…”

Section: Coagulationmentioning

confidence: 99%

Boston city hospital and the thorndike memorial laboratory: the birth of modern haematology

Elrod

Karnad

2003

Br J Haematol

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SUMMARYEstablished in 1923, the Thorndike Memorial Laboratory at Boston City Hospital was the first clinical research laboratory in a municipal hospital in the United States of America. Minot and Castle, who were the second and third directors of the Laboratory, were pioneer haematologists and clinical investigators of the highest calibre who created an atmosphere at the Laboratory that would foster patient-centred research and attract the best physician-scientists to work and train there. The haematology research division of the Laboratory made important original contributions to the understanding of the pathophysiology of anaemia, the mechanisms of red cell and platelet destruction and the phagocytic role of the spleen, the nature of haemoglobin (normal and sickle cell), the nature of haemophilia and its therapy and the early classification of lymphoma. It contributed to the Thorndike Memorial Laboratory's worldwide reputation as a model research laboratory and established its reputation as the birthplace of modern haematology.It is not merely a question of permitting universities to carry on research within the hospital walls, but of actually providing laboratories, equipment, technicians, and salaries for professional workers -it is, in fact, a question of realizing that the fostering of medical research is one of the most important means of enabling a hospital to perform its full function of giving the best medical care to the sick. Francis Weld Peabody (1923)

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Section: Coagulationmentioning

confidence: 99%

Boston city hospital and the thorndike memorial laboratory: the birth of modern haematology

Elrod

Karnad

2003

Br J Haematol

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“…The activity of the activated enzyme is inhibited by plasma fractions containing high concentrations of prothrombin, but there is as yet no proof that prothrombin is itself an inhibitor. The action of the enzyme is definitely proteolytic, since it is capable of digesting fibrinogen, fibrin, gelatin, and casein, as indicated by the progressive formation of non-protein nitrogen when it acts upon these substrates (46,47 (2) thrombin, prepared from the prothrombin which is concentrated in Fraction III-2, by conversion with human placental thromboplastin. Fraction I, purified fibrinogen, and thrombin have all been prepared in dry, stable and sterile form; and they serve as starting materials for the making of a series of products, the properties and uses of which are described in subsequent papers of this series.…”

Section: Fibrinolysinmentioning

confidence: 99%

Chemical, Clinical, and Immunological Studies on the Products of Human Plasma Fractionation. Xv. The Proteins Concerned in the Blood Coagulation Mechanism 12

Edsall¹,

Ferry²,

Armstrong³

1944

J. Clin. Invest.

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The profound biological significance of the clotting of blood needs no emphasis here; the vast number of studies of the process which have been carried out testify sufficiently to its importance, and also show clearly the incompleteness of our knowledge of the underlying mechanisms.4The properties of fibrinogen solutions and of fibrin clots may be profoundly modified in many ways by suitable variations in the conditions of preparation. Thus a wide variety of products is obtainable. In the subsequent papers of this series, the properties and uses of several of these, namely, fibrin clots, fibrinogen plastics, fibrin films, and foams made of fibrinogen and thrombin, will be considered. Here, we shall discuss the properties of certain preparations of protein fractions from human plasma, which may be obtained in active and stable form and which possess specific action on the various aspects of the blood clotting mechanism.The fundamental feature of this mechanism is the transformation of a solution of the protein fibrinogen into the rigid insoluble fibrin clot. This transformation does not occur spontaneously in solutions of suffi8ient purity, but is nor-

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“…Preparation of serum protease was accomplished-by chloroform treatment of the component of serun which is precipitated by dilution and acidification to pH 5.5. This method has been previously described (18). In addition, a component of Fraction (17) of the plasma proteins 2 was found to be a highly active source of the protease, and was also employed.…”

mentioning

confidence: 99%

Studies of Streptococcal Fibrinolysis. Ii. The Inhibition of Streptococcal Fibrinolysis by Antifibrinolysin and Antiprotease 1

Kaplan¹

1946

J. Clin. Invest.

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Studies on Blood Coagulation: A Proteolytic Enzyme Prepared From Calcium and Platelet Free Normal Human Blood Plasma 1

Cited by 47 publications

References 14 publications

Boston city hospital and the thorndike memorial laboratory: the birth of modern haematology

Boston city hospital and the thorndike memorial laboratory: the birth of modern haematology

Chemical, Clinical, and Immunological Studies on the Products of Human Plasma Fractionation. Xv. The Proteins Concerned in the Blood Coagulation Mechanism 12

Studies of Streptococcal Fibrinolysis. Ii. The Inhibition of Streptococcal Fibrinolysis by Antifibrinolysin and Antiprotease 1

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