Studies on Cell Compatibility, Antibacterial Behavior, and Zeta Potential of Ag-Containing Polydopamine-Coated Bioactive Glass-Ceramic

Abstract

Dopamine is a small molecule that mimics the adhesive component (L-DOPA) of marine mussels with a catecholamine structure. Dopamine can spontaneously polymerize to form polydopamine (PDA) in a mild basic environment. PDA binds, in principle, to all types of surfaces and offers a platform for post-modification of surfaces. In this work, a novel Ag-containing polydopamine coating has been developed for the functionalization of bioactive glass-ceramics. In order to study the interactions between the surface of uncoated and coated samples and the environment, we have measured the surface zeta potential. Results confirmed that PDA can interact with the substrate through different chemical groups. A strongly negative surface zeta potential was measured, which is desirable for biocompatibility. The dual function of the material, namely the capability to exhibit bioactive behavior while being antibacterial and not harmful to mammalian cells, was assessed. The biocompatibility of the samples with MG-63 (osteoblast-like) cells was determined, as well as the antibacterial behavior against Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. During cell biology tests, uncoated and PDA-coated samples showed biocompatibility, while cell viability on Ag-containing PDA-coated samples was reduced. On the other hand, antibacterial tests confirmed the strong antimicrobial properties of Ag-containing PDA-coated samples, although tailoring of the silver release will be necessary to modulate the dual effect of PDA and silver.

Preparation of stable and superior flux GO/LDH/PDA‐based nanofiltration membranes through electrostatic self‐assembly for dye purification

“…After adding of positively charged LDH nanoplates, the potential value of GO/LDH membrane shifted forward to −6.2 ± 1.31 mV owing to decrease of the negative charge density of GO surface, which directly indicates the presence of electrostatic interaction between GO and LDH. As to the GO/LDH/PDA/CA composite membrane, the zeta potential value is further negatively shifted (−10.9 ± 2.32 mV), which can be attributed to the contribution of negatively charged PDA under neutral environment …”

“…As to the GO/LDH/ PDA/CA composite membrane, the zeta potential value is further negatively shifted (−10.9 ± 2.32 mV), which can be attributed to the contribution of negatively charged PDA under neutral environment. 51…”

Biosensitive and antibacterial coatings on metallic material for medical applications

“…The relatively high stiffness of BG can cause long‐term stress shielding in high load‐bearing applications (Hench & Jones, 2015). Metallic ions, including silver, strontium, and copper and many more are now incorporated in BG (Rehman, Bastan, et al, 2017; Rehman, Ferraris, et al, 2017; Tejido‐Rastrilla et al, 2019; Yang et al, 2018), and exhibit features, such as antibacterial effects and angiogenesis (El‐Rashidy, Waly, Gad, Röther, et al, 2018; Kaya et al, 2018; Goodman et al, 2013; Williams, 1987).…”

Optimization of Antibacterial Properties of “Hybrid” Metal-Sputtered Superhydrophobic Surfaces

“…We live in the era that bacteria not only develop resistance to antibiotics, but they also genetically acquire it from other strains, becoming so called "superbugs" [5]. Antimicrobial resistance now claims 25,000 deaths per year in the EU [6] and 23,000 in the USA [7], with a total of 700,000 losses globally, estimated to reach 10 million by 2050 [8]. While conventional antibiotics gradually lose their efficacy, there is a dire need for the emergence of alternative methods to tailor material surfaces, rendering them antibacterial so as to halt bacterial colonization and limit the spread of infections.…”