Studies on Cell Division Ii. X-

Spoerl, Edward; Loveless, L. E.; Weisman, T. H.; Balske, R. J.

doi:10.1128/jb.67.4.394-401.1954

Cited by 31 publications

(1 citation statement)

References 5 publications

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“…The dual action of dowex treatment points in the same direction. How these are related to budding and cell separation, found to be independent to some extent by Spoerl et al (6), inhibition of cell division as 4 Wood (8) found that starving a ~haploid strain of Saccharomyces by agitation in M/15 KH2PO4 for 48 hours before irradiation did not alter its radiosensitivity. Although the disagreement between these results and ours may be due to several factors such as strain and ploidy difference, it may also be due to a difference in.…”

Section: Discussionmentioning

confidence: 97%

Effect of Starving and Dowex 50 Treatment on Growth of Normal and X-Irradiated Yeast

Bair

Stannard

1955

The Journal of General Physiology

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The inhibitory effect of x-radiation on cell division is well known and has been demonstrated in yeast many times. The relative insensitivity of other processes, such as respiration and fermentation of glucose, is also well known. However, it has recently been demonstrated by Bait and Stannard (3) that starving (aeration in distilled water), treatment with a cation exchange resin (dowex 50), and/or the absence of K + in the medium could considerably enhance the apparent effect of irradiation on gas exchange. The present investigation was designed to determine whether these treatments had similar effects on the growth of yeast after irradiation. Also the effects of these modifying factors on the growth of normal yeast required investigation.The results showed many similarities and one marked contrast in the effects of these agents on growth processes. Neither caused a permanent alteration in cell division in non-irradiated yeast, Starvation caused some enhancement of radiation effects on both colony formation and growth in a liquid medium. The exchange resin had opposite effects on colony formation and on growth in a liquid medium, actually increasing the per cent of cells able to form colonies after irradiation. These contrasts permitted separation of two effects of irradiation on growth, one a permanent inactivation and the other a temporary delay. Starvation effects were qualitatively similar to those seen on respiration and fermentation. Experimental MetkodsThe yeast used in this investigation was from a pound block of bakers' yeast obtained weekly from Standard Brands, Inc., and cultured 24 hours in a synthetic medium. Culture procedures, harvesting, preparation of suspensions, starving, and dowex 50 treatment (after irradiation) were as described earlier (2, 3). Irradiation was accomplished by exposing a distilled water suspension of yeast, 0.65 mg./ml.

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Section: Discussionmentioning

confidence: 97%