Studies on cell motility in inflammation. I. The chemotactic activity of experimental, immunological and non-immunological, inflammatory exudates

Abstract

The accumulation of leucocytes at the site of inflammation may be brought about by chemotaxis or proliferation in the extravascular tissues. The present paper focuses on the chemotactic properties of different types of experimental inflammatory pleural exudates, using a modified Boyden chamber. The time-course of carrageenan-induced exudate chemotactic activity for polymorphs was maximal at 4 h, thereafter diminishing towards 24 and 48 h. Chemotactic activity for mononuclear cells remained unchanged throughout the 4--48 h time-course. Heating the exudates to 56 degrees C for 1 h partially reduced chemotactic activity. These results correlate well with the migration of polymorph and mononuclear cells into the pleural cavity during carrageenan-induced pleurisy. The potency of polymorph and mononuclear cell chemotactic activity of different exudates was of the following order: carrageenan greater than calcium pyrophosphate greater than reverse passive Arthus greater than dextran. The results are discussed in order to elucidate the differences between the underlying mechanisms responsible for leucocyte accumulation in different types of inflammatory reaction.

Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels

“…(2) The mixed solution was subjected to high-speed centrifugation (MX-301; TOMY, Tokyo, Japan) at 14,000 rpm (17,800 Â g) for 1 h to spin-down colloidal coagulations, and the deposit was removed. (3) The clear sol solution was mildly aspirated to exclude dissolved oxygen, conditioned for 10 min using a deforming agitator (MX-201; THINKY, Tokyo, Japan), and stored at À20 C. The sol solution was warmed at 45 C prior to use for photolithography.…”

“…Organized cell migration in vivo underlies various activities of living tissue, including morphogenetic processes ranging from gastrulation to the development of a nervous system [1], inflammation processes starting from the migration of leukocytes into an area of insult [2,3], and wound-healing processes that involve the migration of fibroblasts and vascular endothelial cells [4]. On the other hand, disordered cell migration can cause diseased tissue reactions such as those seen in tumor metastasis [5], where tumor cells migrate from the initial tumor mass into the circulatory system and spread to new sites.…”

Blood-brain barrier tight junctions are altered during a 72-h exposure to λ-carrageenan-induced inflammatory pain

“…* P Ͻ 0.05 and † P Ͻ 0.01, significantly different compared with controls (0 h). permeability coincides with maximal inflammatory response (33,50).…”

A comparison of the acute inflammatory response in adrenalectomised and sham‐operated rats

“…After 2, 4, 8 and 24 h the rats were killed by exposure to carbon dioxide and the exudates collected. The volumes were measured and the numbers of migrating leucocytes estimated with a Neubauer chamber under light microscopy (Parente et al, 1979;Blackwell et al, 1982). The concentration of eicosanoids in the cell-free exudates were also measured (see previous section).…”