Studies on copper(II) complexes of some polyaza macrocycles derived from 1,2-diaminoethane

Rastogi, Ankur; Nayan, Ram

doi:10.1080/00958970903062046

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…[22][23][24] Different from our previously published protocol, we have used MeOH instead of MeOH/CH 2 Cl 2 17 as solvent for the amidation. This is because CH 2 Cl 2 can react with EDA to generate oligomeric impurities, 25 and at the same time, increase the formation of the defected dendrimer with cyclization terminals. The optimized conditions therefore allow more reliable amidation.…”

Section: Chemical Synthesismentioning

confidence: 99%

Synthesis and use of an amphiphilic dendrimer for siRNA delivery into primary immune cells

et al. 2020

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Genetically manipulating immune cells using siRNAs is important for both basic immunological studies and therapeutic applications. However, the siRNA delivery is challenging because primary immune cells are often sensitive to the delivery materials and generate immune responses. We have recently developed an amphiphilic dendrimer, which is able to deliver siRNA to a variety of cells including primary immune cells. We provide here the protocols for the synthesis of this dendrimer and its siRNA delivery into immune cells such as primary T-and B-cells, natural killer cells, macrophages, and primary microglia. It is noteworthy that the dendrimer synthesis is straightforward, and the siRNA delivery protocol is easy, requiring simple mixing of siRNA and dendrimer in buffer, with subsequent application to the primary immune cells to achieve effective and functional siRNA delivery. This dendrimer-mediated siRNA delivery outperforms largely the standard technique of electroporation, opening a new avenue for functional and therapeutic studies of the immune system. The whole protocol encompasses the dendrimer synthesis which requires 3 weeks, the primary immune cell preparation which takes 3-10 days depending on the tissue source and cell type, the dendrimermediated siRNA delivery and subsequent functional assay which takes additional 3-6 days.

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