A problem has been noted regarding the admixture of fuel to a low viscosity lubricant in hybrid electric vehicles (HEVs). This is very detrimental to the wear and tear of engine operating components. In this study, the operating conditions of HEVs were analysed. Using X-ray fluorescence spectrometry (XRF), engine oils of two different viscosity classes were compared after the operating process and these data were compared with fresh reference samples. Attention was paid to the content of elements such as Ca, Zn, Mo, Sn, Cd, Fe Pb, Si, Cu, and Ni. The depletion of anti-wear additives, as well as the higher content of metallic wear products relative to the operated 5W30 (engine oil), contributed to the overall assessment of the lubricity of the 0W30 oil, as well as to the tribological results. Then, under laboratory conditions, oil samples contaminated with up to 1 to 8% fuel were subjected to rheological (mini AV-X viscometer) and tribological (four-ball tester) tests. The dependence of the local pressure at the metal-to-metal contact point in the kinematic node on viscosity showed the dissimilar nature of the used and fresh oil and the divergence of the domains for the two groups of samples. Increasing the fuel contamination of used oil above 4% drastically reduces the pressure responsible for maintaining the oil film. In order to improve lubricant performance during HEV operation in urban conditions, it was proposed to carry out extra-urban traffic driving in order to evaporate the fuel from the engine oil. A shorter oil change interval is also recommended.