Studies on functional connections between the supraoptic nucleus and the stomach in rats

Lu, Chang-Liang; Li, Zhaoping; Zhu, Jianping; Zhao, Dongqin; Hong-bin, AI

doi:10.1007/s12576-011-0137-z

Cited by 9 publications

(6 citation statements)

References 32 publications

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“…Rats subjected to bilateral destruction of the SN manifest reduced motor coordination accompanied by typical disorders of gastric emptying [11] and alteration of Ach and DA in the DMV [12]. The DMV receives dopaminergic innervation from the NTS [13]. The dopaminergic neurons in the hypothalamus project to the DVC [14].…”

Section: / No Direct Projection Is Observed From the Substantia Nigramentioning

confidence: 99%

No Direct Projection is Observed from the Substantia Nigra to the Dorsal Vagus Complex in the Rat

Wang

Lian

Cai

et al. 2014

Journal of Parkinson's Disease

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Background: Parkinson's disease (PD) is a neurodegenerative disorder that is characterized by degeneration of dopaminergic neurons in the substantia nigra (SN). Destruction of the SN can lead to gastric dyskinesis accompanied by decreased expression of choline acetyltransferase (ChAT) and increased expression of tyrosine hydroxylase (TH) in the dorsal vagus complex (DVC), which includes the dorsal motor nucleus of the vagus (DMV) and nucleus tractus solitarius (NTS). However, it is unclear if the SN and DVC are directly connected. Objective: To investigate the neural projection from the SN to the DVC in rats. Methods: Retrograde and anterograde tracing techniques combined with double-labeling immunofluorescence technique were used. Results: Destruction of the SN significantly decreases ChAT immunoreactivity (IR) and increases TH-IR in the DVC. After injection of the retrograde tracer fluoro-gold (FG) into the DVC, FG-labeled neurons were observed in the hypothalamic paraventricular nucleus (PVN), lateral hypothalamus (LH), inferior olive (IO), and locus coeruleus (LC). No FG-positive cells were observed in the SN or striatum. Furthermore, after injection of anterograde tracer biotinylated dextran amine (BDA) into the SN, BDA-positive fibers were observed in the caudate putamen (Cpu), globus pallidus (GP), LC, and LH but not in the DVC. Conclusion: This study is the first to demonstrate that neurons in the SN do not directly innervate the DVC in rats. The DVC might be indirectly innervated by the SN through the hypothalamus and/or the LC. These data provide important morphological insights into the potential mechanism underlying the gastroparesis observed in PD patients.

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Section: / No Direct Projection Is Observed From the Substantia Nigramentioning

confidence: 99%

No Direct Projection is Observed from the Substantia Nigra to the Dorsal Vagus Complex in the Rat

Wang

Lian

Cai

et al. 2014

Journal of Parkinson's Disease

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“…DMV plays an important role in regulating gastric function (such as gastric acid secretion and gastric motility) Shapiro and Miselis (1985) ; Berthoud et al (1991) . The vagus nerve complex (DMV) can transmit information through the vagus nerve to the stomach, and there is no direct connection between the PVN and the stomach; therefore, we hypothesised that the neurons in the PVN are projected to the DMV and are related to cholinergic neurons innervating the gastric smooth muscle in DMV Lu et al (2011) . We believe that the inhibition of gastric motility may be caused by the inhibitory neurotransmitter VIP or NO released by the vagus nerve to inhibit gastric motility in rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of Exogenous Hydrogen Sulfide in the Hypothalamic Paraventricular Nucleus on Gastric Function in Rats

Sun

Shi

et al. 2022

Front. Pharmacol.

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Background: Hydrogen sulfide (H2S) is a new type of gas neurotransmitter discovered in recent years. It plays an important role in various physiological activities. The hypothalamus paraventricular nucleus (PVN) is an important nucleus that regulates gastric function. This study aimed to clarify the role of H2S in the paraventricular nucleus of the hypothalamus on the gastric function of rats.Methods: An immunofluorescence histochemistry double-labelling technique was used to determine whether cystathionine-beta-synthase (CBS) and c-Fos neurons are involved in PVN stress. Through microinjection of different concentrations of NaHS, physiological saline (PS), D-2-Amino-5-phosphonovaleric acid (D-AP5), and pyrrolidine dithiocarbamate (PDTC), we observed gastric motility and gastric acid secretion.Results: c-Fos and CBS co-expressed the most positive neurons after 1 h of restraint and immersion, followed by 3 h, and the least was at 0 h. After injection of different concentrations of NaHS into the PVN, gastric motility and gastric acid secretion in rats were significantly inhibited and promoted, respectively (p < 0.01); however, injection of normal saline, D-AP5, and PDTC did not cause any significant change (p > 0.05). The suppressive effect of NaHS on gastrointestinal motility and the promotional effect of NaHS on gastric acid secretion could be prevented by D-AP5, a specific N-methyl-D-aspartic acid (NMDA) receptor antagonist, and PDTC, an NF-κB inhibitor.Conclusion: There are neurons co-expressing CBS and c-Fos in the PVN, and the injection of NaHS into the PVN can inhibit gastric motility and promote gastric acid secretion in rats. This effect may be mediated by NMDA receptors and the NF-κB signalling pathway.

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“…These changes may lead to the dysregulation of the autonomic nervous system under many conditions. The large magnocellular neurons in the PVN and SON produce mainly VP and OT as neurotransmitters, which have connections to gastric functions (Flanagan et al, 1992; Lu et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Hypothalamic Astrocytes Respond to Gastric Mucosal Damage Induced by Restraint Water-Immersion Stress in Rat

Sun

et al. 2016

Front. Behav. Neurosci.

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Restraint water-immersion stress (RWIS), a compound stress model, includes both psychological and physical stimulation. Studies have shown that neurons in the hypothalamus are involved in RWIS, but the role of astrocytes and the interactions between astrocytes and neurons in RWIS are not clear. Here, we tested our hypothesis that hypothalamus astrocytes are involved in RWIS and interact with neurons to regulate gastric mucosal damage induced by RWIS. The expression of Glial fibrillary acidic protein (GFAP) and c-Fos in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) significantly increased following the RWIS. GFAP and c-Fos expression are similar in the temporal pattern, peaked at 1 h after the RWIS, then reduced gradually, and reached a maximal level again at 5 h which show “double-peak” characteristics. Intracerebroventricular administration of astroglial toxin L-a-aminoadipate (L-AA) and c-Fos antisense oligodeoxy nucleotides (ASO) both decreased RWIS-induced gastric mucosal damage. Results of immunohistochemistry assay revealed that both L-AA and ASO decreased the activation of astrocytes and neurons in the hypothalamus by RWIS. These results showed that hypothalamus neuron-astrocyte “network” involved in gastric mucosal damage induced by RWIS. This study may offer theoretical basis for some novel therapeutic strategies for RWIS-induced gastric ulcers.

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Studies on functional connections between the supraoptic nucleus and the stomach in rats

Cited by 9 publications

References 32 publications

No Direct Projection is Observed from the Substantia Nigra to the Dorsal Vagus Complex in the Rat

No Direct Projection is Observed from the Substantia Nigra to the Dorsal Vagus Complex in the Rat

Effects of Exogenous Hydrogen Sulfide in the Hypothalamic Paraventricular Nucleus on Gastric Function in Rats

Hypothalamic Astrocytes Respond to Gastric Mucosal Damage Induced by Restraint Water-Immersion Stress in Rat

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