Studies on Human Foetal Haemoglobin. I. Detection and Estimation

Microspectrophotometric Determination of Differentially Extracted Haemoglobin in Single Erythrocytes

“…It is expressed as the percentage of total Hb. In agreement with the findings of J onxis and H uisman (9) and Beaven et al (1) we have found that this pro cedure docs not reveal relative HbF concentrations below 10%. In infants whereno HbF could be demonstrated with the alkali denaturation procedure, we have assumed its concentration to be somewhere in the range below 10% of the total Hb.…”

“…In infants whereno HbF could be demonstrated with the alkali denaturation procedure, we have assumed its concentration to be somewhere in the range below 10% of the total Hb. In normal adults, on the other hand, the relative HbF concentration may be assumed to range between 0 and 1% (1,5).…”

A Simple Method for the Quantitation of Haemoglobin Fractions Obtained by Starch-Gel Electrophoresis

“…The proposed technique which has been utilized in our labora tory for over a year, gives quantitative and analytical results very near to the data reported by many authors, who have used different procedures (1,2,3,4,5,11,12,16,17,20,21,22,23,26,27,29,30,31,32,33,38,39). This method completes the separative electro phoretic technique of haemoglobins on starch-gel : in fact, it makes possible to dose carefully each haemoglobin and protein fraction.…”

Occurrence of Haemoglobin H in Leukaemia: a Further Case of Erythroleukaemia

“…Haemolysates were prepared with out freezing, in order to minimise loss of Hb-H. Starch gel electrophoretic analyses were made at pH 8.6 in Tris-EDTA-borate buffer, and at pH 6.9 in a phosphate buffer [17]. Published procedures [3,24] were used to detect and estimate small proportions of fetal haemoglobin (Hb-F). Transparent starch gels stained with Naph thalene Black 10B were evaluated quantitatively by transmission densitometry [13].…”