Studies on human pancreatic lipase

Colipase enhances hydrolysis of dietary triglycerides in the absence of bile salts.

“…With the complete system there was no discernable lagphase and rate ofhydrolysis was similar to that observed with the triglyceride-gum arabic emulsion (compare Tables I and II), which suggests that it may be close to the "uninhibited" rate for hydrolysis of emulsified long-chain triglycerides. It was previously known that some proteins inhibit the action of pancreatic lipase on emulsified triglycerides (13,14,26), and this is confirmed in our work. Others have shown that this type of inhibition can be relieved by bile salts (14,26).…”

“…It was previously known that some proteins inhibit the action of pancreatic lipase on emulsified triglycerides (13,14,26), and this is confirmed in our work. Others have shown that this type of inhibition can be relieved by bile salts (14,26). Our study demonstrates that this inhibition can also be relieved by colipase.…”

Influence of Colipase on the Turbidimetric Determination of Pancreatic Lipase Catalytic Activity

“…This effect has been noted previously by several authors (6,7,13) and was repeatedly attributed to the presence of specific lipase inhibitors in serum. Four such inhibitors of different molecular weights were fractionated on Sephadex G-200 (7).…”

“…Bile acids äs well äs proteins have repeatedly been reported to be strong inhibitors of pancreatic lipase (9)(10)(11)(12)(13), the mechanism of Inhibition being a competition of these amphiphilic compounds with lipase for the access to the lipid-water interface (11,12). The contradiction between these experimental findings and the fact that lipase physiologically acts in an environment containing high concentrations of bile acids and proteins, was explained during the last deĉ ade by the detection of colipase.…”

Scotland and Northern Ireland Region: Serum Protein Masking of Raised Serum Lipase Levels in Acute Pancreatitis

“…Buffers are not required, the procedure is fully automatic and displays the time course of lipolysis in addition to recording the alkali con-sumed in maintaining a constant pH. In studying human pancreatic lipase and the lipase of pancreatitis serum we have employed a pH-stat technique described by Fraser and Nicol (1966 Although human pancreatic lipase readily hydrolyses emulsified triglyceride substrates, hydrolysis is greatly inhibited by albumin at concentrations in excess of 2 mg.jml., the magnitude of the inhibition being dependent on the order of addition of albumin and enzyme to the substrate (Fraser and Nicol, 1965). Increasing the amount of enzyme in the assay medium does not reverse this inhibition but marked hydrolysis can be elicited by addition of a further aliquot of emulsion substrate (Fraser and Nicol, 1966).…”