Studies on Joint Toxic Effects of a Glyphosate Herbicide (Fozát 480) and a Heavy Metal (Cadmium) on Chicken Embryos

Abstract

The aim of the study was to determine the individual and combined toxic effects ofthe herbicide Fozát 480 (glyphosate [isopropylamine salt] 480g/l) and cadmiumsulphate (CdSO4) on the development of chicken embryos. On the first day ofincubation, chicken eggs were injected with 0.1 ml of cadmium sulphate solution(0.1%) and/or with 0.1 ml of Fozát 480 (2.0%). The chicken embryos wereexamined on the 19th day by measuring the rate of embryo mortality and bodyweight and by identification of different types of developmental anomalies andmacroscopic malformations. The body weight data were statistically evaluated byone-way ANOVA and Dunnett tests, while the embryonic mortality and thedevelopmental anomalies were analysed by Fisher test. Our teratogenicity studyrevealed, that the combined administration of cadmium sulphate and glyphosate(isopropylamine salt) containing herbicide formulation caused a significantreduction in the body weight of embryos and increased the rate of embryonicmortality. The joint toxic effect of cadmium sulphate and Fozát 480 is an additiveeffect compared to the individual toxicity of the test materials.

Effect of in ovo glyphosate injection on embryonic development, serum biochemistry, antioxidant status and histopathological changes in newly hatched chicks

“…Hatchability rate was decreased by injection of Roundup ® compared with the non‐injected control eggs and those receiving 10 mg/kg glyphosate. This difference in survivability can be attributed to the surfactant polyoxyethylene amine (POEA), and other inert ingredients of Roundup ® and not the active compound glyphosate (Szabó et al, ). Several studies that show the majority of toxicity from Roundup ® are directly linked to the presence of the surfactant POEA.…”

“…Also, many experiments for animal have evidenced that glyphosate can affect the embryo development (Ingaramo et al, ). Protection has not only impacted on adult fowls but also on the development of the embryo within the egg (Szabó, Szemerédy, Kormos, Lehel, & Budai, ). The direct exposure of wild fowls’ eggs can be increased, inter alia, by irregular use of the pesticides, by inappropriate spraying techniques and by side drifting of spray liquids (Varnagy & Budai, ).…”

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

“…However, there was a decrease in the hatching rate; an increase in kidney and liver weights and oxidative stress; serum parameter imbalances; histopathological alterations of the kidney, liver, and small intestine; and an increase in the levels of liver function enzymes. Moreover, some perturbations of cytochrome P450 family enzymes were recorded [85,86]. However, embryos exposed from the first day of incubation had a decreased bodyweight with another GBH: Fozat 480 at a higher dose (9.6 g/L).…”

Toxicity Test of Individual and Combined Toxic Effects of Glyphosate Herbicide and Heavy Metals on Chicken Embryos

“…On the base of our teratogenicity studies on single administration of injected glyphosate containing herbicide formulation (Glialka Star) caused a significant reduction in the body weight of embryos and markedly increased the rate of embryonic mortality. The herbicide Fozát 480 (glyphosate) in individual and combined administrations significantly increased the embryonic mortality (Szabó et al, 2017). 42.85 a Significant difference as compared to the control group ( a1 p<0.05; a2 p<0.01).…”