Studies on Liver Messenger Ribonucleic Acid

Trakatellis, Anthony C.; Axelrod, A. E.; Montjar, M.

doi:10.1016/s0021-9258(18)91163-4

Cited by 85 publications

(5 citation statements)

References 24 publications

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“…As a consequence, one m-RNA molecule serves as a template for many polypeptide chains (Villa-Trevino et al, 1964). The half-lives of rat liver m-RNA (Villa-Trevino et al, 1964; Noll et al, unpublished observations) and mouse liver m-RNA (Trakatellis et al, 1964b) have been found to be 48-60 min and 2 hr, respectively. In actinomycinor ethioninetreated animals the rapid m-RNA breakdown without replacement is manifested by a progressive breakdown of polysomes (Staehelin et al, 1963b;Korner and Munro, 1963;Trakatellis et al, 1964a;Villa-Trevino et al, 1964).…”

Section: Discussionmentioning

confidence: 99%

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Trakatellis¹,

Montjar²,

Axelrod³

1965

Biochemistry

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126

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Section: Discussionmentioning

confidence: 99%

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Trakatellis¹,

Montjar²,

Axelrod³

1965

Biochemistry

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126

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“…The reasons for the species specificity were not apparent; they were at first ascribed to differences in lysosomal content. However, since most lysosomal enzymes are not active at the pH used for homogenization, the lytic substances are more likely to be the microsomal nucleases (15), and the advantage in the Charles River mice may have to do with the pathogen-free conditions in which they are raised (17). Subsequent experiments, such as those in this paper, have shown that species differences are much less important when homogenization takes place in isotonic or hypertonic media.…”

Section: Discussionmentioning

confidence: 78%

“…At 4 hr radioactivity largely corresponded with the optical density peaks. In an experiment designed to verify that much of the rapidly labeled 18S RNA was ribosomal, the kidneys labeled for 10 and 20 min were homogenized in 0.88 ~ sucrose without added electrolytes; similar treatment provided maximal separation of rRNA and mRNA in liver homogenates (15). Microsomes and ribosomes were prepared as before (4) and dissolved in 0.5 % SDS.…”

Section: Cytoplasm Totalmentioning

confidence: 99%

Rapidly Labeled Nuclear and Cytoplasmic Renal Rna

Malt

1966

The Journal of Experimental Medicine

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Flux of RNA in whole kidneys is difficult to study because many methods of preparation activate lyric enzymes and because only a little of any isotope administered to an animal is delivered to its kidneys. When data are obtained, their interpretation may be perplexing since renal cells are heterogenous. Nonetheless, Revel and Mandel (1, 2) have demonstrated some differences in the patterns of p82 labeling of renal nuclear and cytoplasmic nucleic acids isolated by column chromatography. We have shown that polyribosomes active in protein synthesis may be obtained from mouse kidneys homogenized in hypotonic buffer in the presence of bentonite, that unilateral nephrectomy causes an absolute increase in the amount of polysomal material in the remaining kidney (3), and that the rate at which rRNA 1 is labeled increases by 2 to 4 times after nephrectomy (4).Experiments to be reported in this paper show that conventional methods of extraction with hot phenol-SDS (5) are not entirely satisfactory for preparation of renal RNA but that a modification that involves stripping nuclear histones in hypertoni c buffer and digesting DNA gel with DNase before extraction (6) permits reproducible and more adequate recovery. Nuclear precursors may be followed into cytoplasmic rRNA and mRNA. Preliminary accounts of this work have appeared (7,8), and a description of metabolic interrelations of the classes of RNA in the renoprival kidney is in progress. Materials and Methods,~nimals were male mice of the Charles River strain (Ham/ICR) between 45 to 52 days old (30 g) caged in groups of 12 or less from the 40th day. Intraperitoneal doses of uridine-6-H 8 (250/~c, 6.5 to 8.2 mc/mmole, New England Nuclear Corp., Boston) were permitted to incorporate for the times indicated in the figures. The mice were killed by spinal transection and the kidneys quickly removed and cooled to 4°C in appropriate buffer.

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“…Other details of the experiment are given in the text. 1961;Penman et al, 1963;Staehelin et al, 1964;Trakatellis et al, 1964). Inspection of Figures 2 and 3 reveals that the specific radioactivity (cpm/z^eo) in this region was relatively high in both tissues, particularly at the shorter incorporation period.…”

Section: Test Of Ribonuclease Activity During the Ltac-mgsoimentioning

confidence: 89%

Pulse-Labeling Patterns of Microsomal Ribonucleic Acid Extracted with Lauryltrimethylammonium Chloride^*

Kuff¹,

Hymer²

1966

Biochemistry

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Studies on Liver Messenger Ribonucleic Acid

Cited by 85 publications

References 24 publications

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Rapidly Labeled Nuclear and Cytoplasmic Renal Rna

Pulse-Labeling Patterns of Microsomal Ribonucleic Acid Extracted with Lauryltrimethylammonium Chloride^*

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Studies on Liver Messenger Ribonucleic Acid

Cited by 85 publications

References 24 publications

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver*

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver*

Rapidly Labeled Nuclear and Cytoplasmic Renal Rna

Pulse-Labeling Patterns of Microsomal Ribonucleic Acid Extracted with Lauryltrimethylammonium Chloride*

Contact Info

Product

Resources

About

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Effect of Cycloheximide on Polysomes and Protein Synthesis in the Mouse Liver^*

Pulse-Labeling Patterns of Microsomal Ribonucleic Acid Extracted with Lauryltrimethylammonium Chloride^*