Studies on marine occurring yeasts: Growth related to pH, NaCl concentration and temperature

Norkrans, Birgitta

doi:10.1007/bf00406719

Cited by 144 publications

(79 citation statements)

References 12 publications

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“…Cryptococcus and Candida species, A. pullulans and C. infirmominiatum (under the previous designation Rhodosporidium infirmio-miniata) have previously been reported from the North Sea (Meyers et al 1967;Meyers and Ahearn 1974;Ahearn and Crow 1980;Crow et al 1977). However, we found D. hansenii and R. mucilaginosa (formerly R. rubra) only after incubation (Table 3), although they were the most abundant species in North Sea water samples in both 1964-1966and 1976(Meyers et al 1967Ahearn and Crow 1980). This could be related to seasonality, as especially D. hansenii was reported to occur with algal blooms and is closely related to high nutrient situations (Meyers et al 1967;Ahearn and Crow 1980).…”

Section: Yeast Community At Helgoland Roadsmentioning

confidence: 79%

“…In pure culture studies, marine yeasts exhibited maximum total growth, highest growth rate, and shortest lag phase at acid pH levels, that is, below pH 7 (Norkrans 1966;Hoppe 1972). This preference for low pH levels leads to the question whether marine fungi will benefit from ocean acidification.…”

Section: Introductionmentioning

confidence: 99%

“…At this site, yeast abundances (viable cell counts) have been recorded from 1964to 1966and from 1980to 1992(Meyers et al 1967Gerdts et al 2004) and additional information on yeast identities based on phenotypic identifications is available for certain periods (Meyers et al 1967;Ahearn and Crow 1980). For our study, a water sample was taken in mid-April 2011 and was incubated in laboratory microcosms at different pH levels (pH in situ [8.10], 7.81 and 7.67).…”

Section: Introductionmentioning

confidence: 99%

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Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

et al. 2013

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Marine yeasts play an important role in biodegradation and nutrient cycling and are often associated with marine flora and fauna. They show maximum growth at pH levels lower than present-day seawater pH. Thus, contrary to many other marine organisms, they may actually profit from ocean acidification. Hence, we conducted a microcosm study, incubating natural seawater from the North Sea at present-day pH (8.10) and two near-future pH levels (7.81 and 7.67). Yeasts were isolated from the initial seawater sample and after 2 and 4 weeks of incubation. Isolates were classified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and representative isolates were identified by partial sequencing of the large subunit rRNA gene. From the initial seawater sample, we predominantly isolated a yeast-like filamentous fungus related to Aureobasidium pullulans, Cryptococcus sp., Candida sake, and various cold-adapted yeasts. After incubation, we found more different yeast species at near-future pH levels than at present-day pH. Yeasts reacting to low pH were related to Leucosporidium scottii, Rhodotorula mucilaginosa, Cryptococcus sp., and Debaryomyces hansenii. Our results suggest that these yeasts will benefit from seawater pH reductions and give a first indication that the importance of yeasts will increase in a more acidic ocean.

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Section: Yeast Community At Helgoland Roadsmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

et al. 2013

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“…Recently, an osmosensing MAP kinase pathway has been shown to be present in the yeast Debaryomyces hansenii (Bansal and Mondal, 2000). This species is considered to be a very highly halotolerant yeast, as it is capable of growing in media containing 4 M NaCl (Adler et al, 1985;Norkrans, 1966;Onishi, 1963). In this article, we report the isolation and characterization of the PBS2 homologue from D. hansenii.…”

Section: Introductionmentioning

confidence: 92%

A PBS2 homologue from Debaryomyces hansenii shows a differential effect on calcofluor and polymyxin B sensitivity in Saccharomyces cerevisiae

Bansal

Sharma

Mondal

2001

Yeast

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The PBS2 gene encodes a MAP kinase kinase that plays a pivotal role in osmosensing signal-transduction pathway in the yeast Saccharomyces cerevisiae. Mutation in the PBS2 gene has a pleotropic effect. Besides being osmosensitive, pbs2 mutants show altered sensitivity to polymyxin B and calcofluor. Recent studies revealed that Pbs2p plays a different role in osmoadaptation and calcofluor sensitivity. We have isolated a gene homologous to PBS2 from the highly salt-tolerant yeast Debaryomyces hansenii by phenotypic complementation. DNA sequencing of the clone revealed that the gene encoded a protein of 683 amino acid residues. Like Pbs2p, this protein also has a proline-rich motif. Further characterization revealed that this gene could complement polymyxin B sensitivity but did not affect calcofluor sensitivity. Thus, it appeared that Pbs2p also has an independent role in these two physiological processes. The

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“…NORKRANS [16] relatou que 4% de sal reduz o crescimento de Saccharomyces cerevisae em torno de 10-15%, quando comparados com o controle (sem sal) e que 8% de NaCl reduz a biomassa em 90%. RICHMOND [19], estudando o efeito de altas concentrações de sal na produção de L-lisina, observou que o Nacl a 1% inibe o crescimento de Pediococcus acidilactici.…”

Section: -Introduçãounclassified

Efeito do cloreto de sódio na produção de proteínas (Saccharomyces cerevisiae) em fermentação semi-sólida

Rodrigues¹,

Sant’Anna²

2001

Ciênc. Tecnol. Aliment.

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* A quem a correspondência deve ser enviada. RESUMOEstudou-se o efeito do cloreto de sódio sobre a produção de biomassa e proteínas extracelulares totais, durante o cultivo de Saccharomyces cerevisiae. A levedura foi desenvonvida em fermentador de leito fluidizado, com vazão de ar de 70L/min, temperatura de 33° C, e umidade relativa de 99-100%. Foi utilizado substrato semi-sólido de batatas, previamente hidrolizado, acrescido de cloreto de sódio 0,6M. O crescimento celular foi monitorado por densidade óptica à 595 nm. Observou-se, como resultado, que a adição de cloreto de sódio 0,6M induziu um aumento de 36,86% na produção de proteínas extracelulares totais, mas inibiu o crescimento celular em 27,62% quando os meios com e sem cloreto de sódio foram testados. A produção máxima de biomassa, tanto para os experimentos com adição de cloreto de sódio quanto para o sem adição, ocorreu no perío-do de 7 a 9 horas de fermentacão, enquanto que a produção de proteínas extracelulares totais, independentemente da adição do sal, ocorreu durante o período de 9 a 12 horas de fermentação. As velocidades específicas máximas de crescimento foram de 0,350/h para os experimentos com sal, e de 0,339/h para aqueles sem a adição do sal. A combinação de alta vazão de ar e a presença de cloreto de sódio 0,6M na fermentação parece não ter tido efeito sobre a duração da fase lag na curva de crescimento celular de Saccharomyces cerevisiae. Palavras-chave:Fermentação em leito fluidizado, Saccharomyces cerevisiae, proteínas extracelular. SUMMARY EFFECT OF SODIUM CHLORIDE ON PROTEIN PRODUCTION (Saccharomyces cerevisae) BY SEMI-SOLID FERMENTATION.The effect of sodium chloride on the cell's growth and total extracellular protein production during fermentation of Saccharomyces cerevisiae in an air-fluidized bed fermentation, with a 70 L/min air flow at 33° C and 99-100% relative unidity was studied. A semi-solid potato substrate (previously hydrolized) with 0.6M sodium chloride was used. Cell's growth was monitored by optical density at 595 nm. Results showed that the addition of 0.6M sodium chloride enhanced total extracellular protein level (36.86%). On the other hand, the addition of sodium chloride inhibited cell growth (27.62%), when mediums with and without sodium chloride were tested. The maximum yield of biomass was achieved at the 7 -9th hour fermentation for experiments either with or without addition of sodium chloride, while maximum yield of total extracellular proteins was achieved at the 9 -12th hour fermentation, in all runs. The maximum specific cell's growth rate averaged 0.350/h for the experiments where salt was added and 0.339/h for those without salt. Finally, it seemed that the length of the lag phase had not been affected by the combination of high air flow rate and the presence of 0.6M sodium chloride in the yeast growth curve in this air-fluidized bed fermentation of Saccharomyces cerevisae.Keywords: Air-fluidized bed fermentation, Saccharomyces cerevisae, extracellular protein. -INTRODUÇÃOProteínas unicelulares, princip...

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Studies on marine occurring yeasts: Growth related to pH, NaCl concentration and temperature

Cited by 144 publications

References 12 publications

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

Study on the effects of near-future ocean acidification on marine yeasts: a microcosm approach

A PBS2 homologue from Debaryomyces hansenii shows a differential effect on calcofluor and polymyxin B sensitivity in Saccharomyces cerevisiae

Efeito do cloreto de sódio na produção de proteínas (Saccharomyces cerevisiae) em fermentação semi-sólida

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