Studies on oestradiol receptors in uterus

Rochefort, Henri; Alberga, Audrey; Truong, H; Baulieu, Étienne-Émile

doi:10.1042/bj1150045pb

Cited by 4 publications

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“…is the only causative agent for the early action of the hormone in vivo, it should be possible to simulate its effects on the uterine metabolism in vitro. Oestradiol is taken up readily by the uterus both in vivo and in vitro and is found bound to macromolecular receptors in the high speed supernatant fraction and nuclei in both cases (Jensen & Jacobson, 1962 ;Talwar, Segal, Evans & Davidson, 1964 ;Stone & Baggett, 1965a, b;Talwar, Segal, Gupta, Querido, Kakar, Nandi, Srinivasan & Sopori, 1965;Jensen, Jacobson, Flesher, Saha, Gupta, Smith, Colucci, Shiplacoft, Neumann, De Sombre & Jungblut, 1966;Jensen, De Sombre & Jungblut, 1967;Jensen, Suzuki, Kawashima, Stumpf, Jungblut & De Sombre, 1968;Baulieu, 1969;Erdos, Bessada& Fries, 1969;King, Gordon & Steggles, 1969;Rochefort, Alberga, Truong & Baulieu, 1969;Shyamala & Gorski, 1969). Furthermore, over 95% of the steroid remains chemically unchanged in the uterus and is extractable as oestradiol-17/?, so that no chemical transformation of the hormone to an alternate active compound appears to be required.…”

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confidence: 99%

ACTION OF OESTRADIOL-17β AND CYCLIC AMP ON THE SYNTHESIS OF RNA, PHOSPHOPROTEINS AND PHOSPHOLIPIDS IN THE UTERUS OF OVARIECTOMIZED RATS IN VITRO

Rao¹,

Gp²

1972

Journal of Endocrinology

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Administration of oestradiol-17\g=b\ to ovariectomized rats increases the incorporation of [32P]orthophosphate into RNA, phosphoproteins and phospholipids of the uterus. Uteri taken from the ovariectomized rats pretreated with oestradiol for 1 h in vivo continued to manifest enhanced ability to incorporate 32P into RNA, phospholipids and phosphoproteins when tested in vitro. However, when oestradiol was added directly to the incubation medium, it failed to stimulate to the same extent the incorporation of [32P]orthophosphate into RNA and phosphoproteins in excised uteri from ovariectomized rats. An increase in the incorporation of 32P into phospholipids was obtained only after prolonged incubation of the excised uteri in the medium containing oestradiol.A number of experimental approaches has been tried to reproduce more completely the in-vivo actions of oestradiol on the rat uterus in vitro. Supplementation ofthe medium with hormones such as insulin, progesterone and biogenic amines like adrenaline, histamine and serotonin besides oestradiol was not sufficient to attain the metabolic capacities of the uterus from animals primed with oestradiol in vivo. Oestrone was not effective. The presence of tissues such as liver, parametrial fat, adrenal glands or anterior pituitary in the incubation medium did not modify the lack of full response of ovariectomized rat uteri to oestradiol in vitro.Studies were also extended to uteri incubated in the peritoneal cavity of ovariectomized rats instead of in synthetic media. Moreover the uteri of rats in pro-oestrus and dioestrus were also tested in vitro for their lack of full invivo-like response to oestradiol. Cyclic 3\m=' \,5\m=' \-AMP and AMP stimulated in vitro the incorporation of [32P]orthophosphate into RNA but not into phosphoproteins and phospholipids.Results are discussed and arguments made for the lesion possibly preventing the full development of the uterotropic action of the hormone in vitro, even though limited effects of the hormone were obtained.

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