Studies on optimization of L-glutaminase production under submerged fermentation from marine Bacillus subtilis JK-79

Abstract

In the current study, a marine strain Bacillus subtilis JK-79 capable of producing L-glutaminase enzyme, was analyzed for maximal L-glutaminase production. The Plackett-Burman design (PBD) was applied to determine the significant variables. Optimum conditions of the significant variables on Lglutaminase production were determined by the Central Composite Design (CCD) of response surface methodology. Maximum L-glutaminase production of 691.27 U/ml under flask condition was obtained at the predicted optimal values of pH 6.9, fructose 2.1% (w/v), yeast extract 1.25% (w/v), and glutamine 2.47% (w/v). Statistical optimization has enhanced L-glutaminase enzyme production upto 3.48 fold when compared with the basal SWG medium. The results of this study revealed that marine B. subtilis JK-79 could be a promising source for L-glutaminase production.