Studies on Physiological Effects and Plasmid Stability of JM109 Host System

Abstract

Objective: The purpose of the study delineates the growth and plasmid stability of E. coli JM109 host system.
 Methods: Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability.
 Results: Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, dantron showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth was observed in exposure to UV rays. Conclusion: The present work discussed that, the effect of drugs, chemicals, radio frequency and microwave radiation have a huge effect not only on growth of organism but also concentration and stability of plasmid.

Study on the Plasmid and Genomic DNA Stability of DH5α Host System

“…Plasmids have a significant impact on productivity, it is necessary to conduct research on plasmid stability and colony forming units. at both the industrial and research levels [4]. Plasmids can also perform additional functions, such as plasmid-encoded genes that can improve the host's survival by completely killing a different organism or synthesizing toxins that act as defensive mechanisms.…”