Studies on PIVKA VII

Mariani, G.; Mazzucconi, M.G.; Solinas, S; Avvisati, Giuseppe; Chistolini, Antonio; Moretti, T

doi:10.1159/000215063

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…C appears to reflect a decrease of synthesis of the protein, in association with reduced levels of the other vitamin K-dependent proteins. In long-term dicoumarol-treated patients, the discrepancy between F VII Ag and F VII C is in favor of the presence of a-and/or hypocarboxylated molecules as previously reported for F VII (24)(25)(26), and for other vitamin K-dependent proteins (16-30). In two patients tested at the onset of anticoagulant therapy, there was a rapid disappearance of both F VII and protein C antigens, indicating a similar short (5-7 h) half-life for both proteins, in accordance with another report (31).…”

Section: Discussionsupporting

confidence: 76%

An Enzyme Immunoassay (ELISA) for the Quantitation of Human Factor VII

et al. 1986

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SummaryA new solid phase enzyme-linked immunosorbent assay (ELISA) was developed for the quantitation of human Factor VII antigen (F VII Ag), using a monospecific rabbit anti-F VII antiserum. Anti-F VII F(ab′)2 fragments were adsorbed to polystyrene plates. The binding of serial dilutions of control or test plasma, containing F VII, was detected by incubation with peroxidase-labeled anti- FV II IgG followed by the addition of hydrogen peroxyde and O-phenylenediamine. This ELISA is specific, sensitive (detection limit: 0.05%) and accurate (coefficient of variation: 1.5-4% for within- and 1.6-9% for between-assays). F VII coagulant activity (F VII C) and F VII Ag were determined in large populations of controls and patients. In normal plasma (n = 38), F VII Ag ranged from 83 to 117% and the correlation coefficient between F VII Ag and F VII C was 0.94. In patients with severe (F VII C inf. 1%) congenital F VII deficiency (n = 5), F VII Ag was undetectable in two cases (inf. 0.05%) and markedly reduced (0.35 to 5.6%) in the three other cases. In patients with liver cirrhosis (n = 15), F VII Ag ranged from 21 to 59% and was in good correlation with F VII C (r = 0.84). In dicoumarol treated patients (n = 15), the levels of F VII Ag ranged from 51% to 79% and a poor correlation (r = 0.52) with F VIIC was observed. In “compensated” DIC (n = 5), levels of F VII Ag varied from 60 to 186%, with significantly higher F VII C levels (from 143 to 189%). In contrast, in “decompensated” DIC (n = 7), low F VII Ag and F VII C levels were observed (from 7 to 27%). In patients with deep-vein thrombosis (n = 25), high levels of F VII Ag (from 102 to 136%) and F VII C (from 110 to 150%) were demonstrated. In surgical patients, no significant difference was observed before and one day after intervention.

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Section: Discussionsupporting

confidence: 76%

An Enzyme Immunoassay (ELISA) for the Quantitation of Human Factor VII

et al. 1986

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“…More specific tests are required to demonstrate vitamin K deficiency, such as the measurement of abnormal, noncarboxylated prothrombin by enzyme-linked sandwich immuno-assay, using a monoclonal antibody against this abnormal prothrombin (11,12). Determination of the concentration of other precursor proteins (PIVKA, IX, X, VII) by specific antibodies could give additional information (10,14).…”

Section: Discussionmentioning

confidence: 99%

Protein C Levels in Infancy and Early Childhood

Widdershoven

Bertina²,

Monnens

et al. 1987

Acta Paediatrica

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Protein C antigen levels were measured in the plasma of healthy full term infants by electroimmunoassay. During the first three months of life (on day four, at one month, two months and three months of age) protein C antigen levels were compared in breast-fed and bottle-fed infants. None of the two groups of infants received vitamin K at birth. Only at the age of three months there was a significant difference between the groups. Unexpectedly infants, who were breast-fed, had a higher protein C level at three months of age. Levels were also measured in 15 healthy children between one and three years of age. The antigen levels increase with age to reach adult values at about three years of age.

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“…Based on reactivity with different thromboplastins, Girolami et al [2][3][4][5] and Girolami [6] reported three activa tion variants: Padua 1, Padua 2 and Verona. On the other hand, using a neutralization assay several molecular variants of FVII were described [7], Mariani et al [8][9][10] clas sified FVII deficiency according to the rela tionship between FVII coagulant activity (FVIIC) and FVII related antigen (FVIIAg) levels, and established three immunological variants: VII-, VII+, and VIIR.…”

Section: Introductionmentioning

confidence: 99%

Study of Different Factor VII Deficiency Variants in Nine Families from Spain

Pardo

Oteyza²,

Blanco³

et al. 1987

Pathophysiol Haemos Thromb

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Twenty-three patients with congenital factor VII (FVII) deficiency, belonging to 9 kindreds were studied. Immunological variants were classified according to the relationship between FVII coagulant activity (FVIIC) and the level of FVII antigen (FVIIAg), considering 3 previously described groups: VII-, VII+ and VIIR. Activation variants were determined by the reactivity pattern with three different thromboplastins. One patient was classified as VII-, 16 as VII+, and 6 as VIIR. Three patients belonging to the same kindred showed a Padua 2 FVII deficiency, and 1 patient showed a Padua 1 variant. There was no correlation between antigenic or activation variants and severity of bleeding tendency. Classical autosomal recessive mode of inheritance was observed in VII- and VII+ families. Nevertheless, a possible autosomal dominant trait was observed in VII+ kindreds.

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Studies on PIVKA VII

Cited by 4 publications

References 18 publications

An Enzyme Immunoassay (ELISA) for the Quantitation of Human Factor VII

An Enzyme Immunoassay (ELISA) for the Quantitation of Human Factor VII

Protein C Levels in Infancy and Early Childhood

Study of Different Factor VII Deficiency Variants in Nine Families from Spain

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