Studies on platelet aggregation using the Born method in normal and uraemic dogs

Mischke, R.; Schulze, Ursula

doi:10.1016/j.tvjl.2004.02.009

Cited by 26 publications

(16 citation statements)

References 20 publications

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“…The turbidimetric method used in the present study has greater clinical relevance and it is easier to standardize than the electrical impedance method. Other agonists can be used, such as ristocetin and epinephrine, but dogs platelets are much less responsive to these substances than human platelets; therefore, ADP is the first choice agonist for platelet aggregation test in dogs [21]. …”

Section: Resultsmentioning

confidence: 99%

Hemostatic profile and thromboembolic risk in healthy dogs treated with prednisone: a randomized controlled trial

et al. 2013

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BackgroundThrombosis has been associated to some diseases like hyperadrenocorticism (HAC). Several drugs can alter the balance, such as the corticosteroid prednisone, used mainly for its anti-inflammatory and immunosuppressive effects. It is known that hypercortisolism can stimulate thrombi formation by increasing coagulation factors and decreasing fibrinolysis. However it is not known how prednisone administration affects hemostasis in dogs and if it is dose dependent. The aim of this study, therefore, was to demonstrate the effects of prednisone administration on dogs’ hemostatic profile.ResultsSignificant decrease of antithrombin levels was observed in both groups (anti-inflammatory and immunosuppressive doses) after 15 days of treatment. An increase of platelet aggregation was observed in dogs receiving immunosuppressive doses of prednisone (Group II).ConclusionsFrom the results obtained in our study, it is not possible to infer that hypercortisolism can increase the thromboembolic risk, despite the decreased anticoagulant factors (antithrombin levels).

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Section: Resultsmentioning

confidence: 99%

Hemostatic profile and thromboembolic risk in healthy dogs treated with prednisone: a randomized controlled trial

et al. 2013

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“…In a canine study using the Born method, the following threshold concentrations (final test concentrations) were established: 25 µmol/l adenosine diphosphate (ADP), 10 µg/ml collagen and 1 IU/ml thrombin (Mischke and Schulze, 2004). In an actual study using the Multiplate instrument, the following optimal agonist concentrations (sourced from Roche Diagnostics GmbH) were defined: 10 µmol/l ADP, 5 µg/ml collagen, and 1 mmol/l arachidonic acid (kalbantner et al, 2010).…”

Section: Platelet Function Analysismentioning

confidence: 99%

Laboratory evaluation and interpretation of haemostasis in small animals

Mischke

2017

J Hellenic Vet Med Soc

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This review deals with indications for performing haemostasis tests, methodical aspects of blood sampling and handling of sample material, selection of appropriate tests and basic principles of test interpretation. Indications for laboratory evaluation of haemostasis in small animals include spontaneous bleeding or bleeding disproportionate to the degree of trauma, bleeding from multiple sites and diseases which are frequently associated with haemostatic disorders. Patient history and clinical findings can deliver clues for the type of haemorrhagic diathesis. Important preanalytical issues include prevention of prolonged venous stasis, order of drawing and appropriate filling of the collection tubes and careful mixing of blood with anticoagulant. Initial screening can be performed by global tests of haemostasis including viscoelastic testing using thrombelastography and rotation thrombelastometry and/or a "basic examination profile" including platelet count, capillary bleeding time (optional in cases of suspected functional platelet disorders) and group tests prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin (clotting) time (optionally in cases of prolonged PT and APTT). This procedure allows a rational evaluation of the haemostatic system and a specific use of further tests including individual coagulation factor activities, inhibitor activities, activation markers, examination of von Willebrand factor, and platelet function analyses. It is important (1) to use species-and method specific reference values for interpretation and (2) to consider the limitations of tests when performed according to the standard test procedure optimised for human sample material (e.g., low sensitivity of PT test for canine and feline samples).

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“…35,48 Animals with hypothyroidism may have mild platelet dysfunction, but the origin of this change is unclear and of questionable clinical import. 49 -51 In animals with severe uremia that require anesthesia, the same precautions listed previously for patients with thrombocytopenias are relevant.…”

Section: Thrombocytopathiasmentioning

confidence: 99%