Studies on properties of surfaces required for growth of mammalian cells in synthetic medium

Rappaport, Catherine; Poole, Jenna; Rappaport, Harry P.

doi:10.1016/0014-4827(60)90117-8

Cited by 102 publications

(18 citation statements)

References 6 publications

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“…Paramoeba was often seen in large clumps in such areas. Similar observations have been recorded for other cell types(24). Divalent cations were not required for adhesion ofParamoeba to polystyrene surfaces, as amoebae adhered well in CMF alone.…”

supporting

confidence: 87%

“…Once sufficient contact between cell and substrate has occurred, charge density exerts little effect on Paramoeba, as morphology and locomotive rates in each medium do not differ between treated and untreated dishes or culture tubes. In the case of glass substrata, sodium ions, which are complexed with SiO-residues, are ionized when in contact with liquid media; hence, the greater the soda (Na,O) content of glass, the greater the negative surface charge density of the glass (24). Thus, if negative charge density were strictly dependent on substrate composition, slides and cover slips should give a low percent transmission (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Adhesion, Morphology, and Locomotion of Paramoeba pemaquidensis Page (Amoebida, Paramoebidae): Effects of Substrate Charge Density and External Cations1

Martin

1987

The Journal of Protozoology

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Morphology and locomotive behavior in the marine amoeba, Paramoeba pemaquidensis Page, was examined under different environmental conditions. Paramoeba requires a minimum surface negative charge density for adhesion of amoebae to substrata. Once adhesion to the substratum has been attained, however, surface negative charge density has no effect on morphology or locomotive rate. Divalent cations are not required for adhesion, but external calcium is required for normal locomotion. In the presence of calcium, Paramoeba often assumes a locomotive form with a broad, well‐developed anterior hyaline region and truncate posterior region. Locomotive forms vary from those with only a well‐developed hyaline region (Flabellula‐like) to forms with long digitiform sub‐pseudopodia (Vexillifera‐like), with intermediate morphotypes. Locomotive rates decrease and anteroposterior polarity disappears in the presence of living or heat‐killed bacteria, indicating that phagocytosis temporarily interferes with locomotion and alters form.

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supporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Adhesion, Morphology, and Locomotion of Paramoeba pemaquidensis Page (Amoebida, Paramoebidae): Effects of Substrate Charge Density and External Cations1

Martin

1987

The Journal of Protozoology

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“…The idea that sterilization may affect cell adhesion by causing changes in the glass surface has been introduced in 1965, 12 based on observations of poor fibroblast attachment to Pyrex glass pre-treated with NaOH. 13 Still, there is no clear understanding of the specific changes in glass surfaces introduced by alkali, acid or water-steam treatment, and of heating and sterilizing procedures in general. A clear correlation between adhesion strength of mammalian cells and the nanoroughness of glass following sterilization treatment has also not been established so far.…”

Section: Discussionmentioning

confidence: 99%

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

Wittenburg

Lauer

Oswald

et al. 2013

J Biomedical Materials Res

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Producing sterile glass surfaces is of great importance for a wide range of laboratory and medical applications, including in vitro cell culture and tissue engineering. However, sterilization may change the surface properties of glass and thereby affect its use for medical applications, for instance as a substrate for culturing cells. To investigate potential effects of sterilization on glass surface topography, borosilicate glass coverslips were left untreated or subjected to several common sterilization procedures, including low-temperature plasma gas, gamma irradiation and steam. Imaging by atomic force microscopy demonstrated that the surface of untreated borosilicate coverslips features a complex landscape of microislands ranging from 1000 to 3000 nm in diameter and 1 to 3 nm in height. Steam treatment completely removes these microislands, producing a nanosmooth glass surface. In contrast, plasma treatment partially degrades the microisland structure, while gamma irradiation has no effect on microisland topography. To test for possible effects of the nanotopographic structures on cell adhesion, human gingival fibroblasts were seeded on untreated or sterilized glass surfaces. Analyzing fibroblast adhesion 3, 6, and 24 h after cell seeding revealed significant differences in cell attachment and spreading depending on the sterilization method applied. Furthermore, single-cell force spectroscopy revealed a connection between the nanotopographic landscape of glass and the formation of cellular adhesion forces, indicating that fibroblasts generally adhere weakly to nanosmooth but strongly to nanorough glass surfaces. Nanotopographic changes induced by different sterilization methods may therefore need to be considered when preparing sterile glass surfaces for cell culture or biomedical applications.

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“…Since HeLa cells were first established in vitro as a strain readily grown by serial subculture (1, 2) they have been exceptionally widely used for the propagation of viruses and for a variety of other purposes such, for example, as the study of cell adherence (3,4) and mitosis (5), the assay of toxic substances (6), and the investigation of biosynthetic processes (7). However, despite the ubiquity of HeLa ceils in many types of laboratory, relatively little has been reported concerning their fine structure under normal conditions of culture, the electron microscope having mainly been used to study them after infection with various viruses (8)(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

Epstein

1961

The Journal of Cell Biology

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HeLa cells from conventional culture media have been studied in thin sections with the electron microscope; in many cases cells were examined in sets of sections cut in series. The fine structure of the cells is described including three unusual features not hitherto reported. It has been found that numerous cells contained rows of parallel smooth surfaced cisternae spaced about 150 m# apart and communicating with rough surfaced elements of the endoplasrnic reticulum. These cisternae resembled "annulate lamellae" but did not contain regular arrays of pores. In many cells an area of juxtanuclear cytoplasm was occupied by a membranous structure composed of closely applied pairs of narrow cisternae either arranged in concentric rings or else extending in several directions in a haphazard manner. Sparse particles were present on the outer membranes of each pair of cisternae. Communications between the double cisternae and other membrane-bounded structures were not observed. A small number of cells contained areas of cytoplasm devoid of organelles and filled with amorphous fuzzy material. The observations recorded are discussed.

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Studies on properties of surfaces required for growth of mammalian cells in synthetic medium

Cited by 102 publications

References 6 publications

Adhesion, Morphology, and Locomotion of Paramoeba pemaquidensis Page (Amoebida, Paramoebidae): Effects of Substrate Charge Density and External Cations1

Adhesion, Morphology, and Locomotion of Paramoeba pemaquidensis Page (Amoebida, Paramoebidae): Effects of Substrate Charge Density and External Cations1

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

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