Studies on protein synthesis in eukaryotic subcellular systems

The dissociation of free ribosomes at elevated concentrations of KCl is dependent on hydrostatic pressure. The pressure necessary for the dissociation is determined for KCl concentrations ranging from 0.1-0.4 M. It varies between 425 kg cm(-2) at 0.1 M and 10 kg cm(-2) at 0.4 M. The partial dissociation of complex ribosomes in KCl is dependent on hydrostatic pressure in the same way as the complete dissociation of free ribosomes. Therefore, it is concluded that mRNA and peptidyl-tRNA do not contribute to the stability of the ribosome under these conditions.

show abstract

The puromycin reaction mediated by yeast ribosomes in high salt

Mast

Bloemers

1973

Molecular Biology Reports

View full text Add to dashboard Cite

The extend of the reaction between puromycin and yeast peptidyl-tRNA prelabeled in vitro was determined by measuring the distribution of trichloroacetic acid precipitable material in isokinetic sucrose gradients in the presence of 0.5 M KCl.Thus it was found that increasing amounts of puromycin remove increasing amounts of peptidyl-tRNA from the 80S position in the gradient. The extend of the reaction, however, was independent of pretreatment of the ribosomes with inhibitors of the translocation indicating that peptidyl-tRNA at the donor and at the acceptor site of the ribosomes are equally accessible to puromycin at 0.5 M KCl.The exposure of both ribosomal binding sites to puromycin in high salt is accompanied by an enhanced reactivity of puromycin towards peptidyl-tRNA. The ED50 determined by measuring the inhibition by puromycin of the poly-U dependent phenylalanine incorporation drops from 5×10(-5) M below 250 mM KCl to 5×10(-6) M at 300 mM and higher concentrations of KCl.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Studies on protein synthesis in eukaryotic subcellular systems

Cited by 5 publications

References 31 publications

Requirements for the initiation of polyphenylalanine synthesis by recombined ribosomal subunits from yeast

Requirements for the initiation of polyphenylalanine synthesis by recombined ribosomal subunits from yeast

Dissociation of yeast ribosomes; The effects of hydrostatic pressure and KCl

The puromycin reaction mediated by yeast ribosomes in high salt

Contact Info

Product

Resources

About