Studies on Siderophore and Pigment Produced by an Adhered Bacterial Strain Halobacillus trueperi MXM-16 from the Mangrove Ecosystem of Goa, India

Kharangate-Lad, Amrita; Bhosle, Saroj

doi:10.1007/s12088-016-0591-7

Cited by 24 publications

(10 citation statements)

References 21 publications

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“…In addition, the biodiversity of soil microbes is a useful indicator of ecosystem change (Castaneda and Barbosa 2017). Though there is an upsurge in interest on bacterial diversity of mangrove sediment in recent years, most of the studies on mangroves of Goa relied on the study of individual microorganisms through culture-based assays (Poharkar et al 2016;Kharangate and;Bhosle 2016). In modern era of scientific advancements, 'omic' technologies are emerging as potential tools to ensure holistic insight into environmental systems, thereby leading to in-depth understanding of the complex mechanisms in an ecosystem (Sharma and Lal 2017).…”

Section: Introductionmentioning

confidence: 99%

Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis

Haldar

Nazareth

2018

3 Biotech

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The present study compared the taxonomic diversity and evaluated the functional attributes of the bacterial species from Mandovi and Zuari mangrove sediments, Goa, using paired-end amplicon sequencing of 16S rDNA and culture-based analyses, respectively. 16S rDNA sequencing revealed Proteobacteria, Firmicutes, and Actinobacteria as the dominant phyla in both the sediments. However, the abundance of these phyla significantly differed between the samples. Bacteroidetes from Mandovi sediment, and Acidobacteria and Gemmatimonadetes from Zuari sediment were the other exclusive major phyla. Chloroflexi, Cyanobacteria, Nitrospirae, Planctomycetes, Verrucomicrobia, and WS3 were the minor phyla observed in both. However, a significant difference in the distribution of minor phyla and lower bacterial taxa under each phylum was noted between the sediments, indicating that the resident microbial flora completely differed between them. This was further validated by high values from distance matrix analyses between the samples. In addition, the pathogenic Vibrio sp. was recorded exclusively in Mandovi sediment, while higher abundance of ecologically important bacterial classes including Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, and Bacilli was observed in Zuari sediment. Taken together, the data indicated that Zuari sediment was taxonomically richer than Mandovi sediment, while a greater incidence of anthropogenic activities occurred in the latter. This observation was further validated by non-parametric richness estimators which were found to be higher for Zuari sediment. The cultured bacterial isolates, all identified as Firmicutes, were tested for activities related to biofertilization and production of enzymes to be used for bioremediation and chemotherapeutic applications. Higher number of bacterial isolates from Mandovi was found to produce indole-acetic-acid, tannase, xylanase, and glutaminase enzymes, and could solubilize phosphate. In contrast, higher proportion of bacterial isolates from Zuari sediment were capable of producing amylase, cellulase, gelatinase, laccase, lipase, protease, and asparaginase enzymes, emphasizing the fact that the Zuari mangrove sediment is a rich reservoir for economically and biotechnologically important bacterial species.

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Section: Introductionmentioning

confidence: 99%

Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis

Haldar

Nazareth

2018

3 Biotech

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“…For example, isolates 27 and 28 inhibited the growth of five test cultures of bacteria and fungi at the same time. Although the activity of most actinomycetes did not exceed AI 6, some isolates (27,28,32) showed a high level of antimicrobial action with AI 30,0 -32,0 against P. savastanoi pv. phaseolicola and X. campestris pv.…”

Section: Resultsmentioning

confidence: 93%

“…Producers of siderophores were determined on the YEM medium. On 7th growth day the colonies were flooded by CAS-indicator solution (Verma et al, 2012) and incubated for 1 h at room temperature. Colonies that synthesized siderophores changed the color of medium from blue to yellow, light yellow or purple.…”

Section: Methodsmentioning

confidence: 99%

Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica E. Desv. (Galindez Island, Antarctica)

Tistechok

Skvortsova

Luzhetskyy

et al. 2019

UAJ

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Aim. The isolation of actinomycetes from Deschampsia antarctica É. Desv. (Galindez Island, Antarctica) rhisosphere and determine their ability to produce compounds with antimicrobial and plant growth promotional properties. Methods. Actinomycetes were isolated using the three different methods: direct inoculation, the roots treatment with an aqueous solution of phenol and heated at 100 °C for 60 minutes. To study the antibacterial activity of actinomycetes, they were plated on the Oatmeal medium and flooded by 0,7% agar with specific test-culture. Antifungal activities were studied by putting the agar block with fungal culture on Petri plates with cultures of actinomycetes. An activity index was determined by the ratio of the diameter zone of the inhibit growth test-cultures to the diameter of the actinomycetes colonies. Plant growth promotion properties were studied by commonly accepted methods. Results. 35 psychotolerant isolates were identified among the 43 actinomycetes isolated from D. antarctica rhizosphere. Almost 42% of actinomycetes isolates were antagonists at least one of typical strain of phytopathogenic bacteria (Pseudomonas savastanoi pv. phaseolicola, Xanthomonas campestris pv. campestris, Agrobacterium tumifaciens, Erwinia amylovora) or fungi (Fusarium oxysporum, Botrytis cinerea, Alternaria alternata). An activity index of most isolates was 3-6, in some isolates-30-32. The potential plant growth promotion properties of isolates were evaluated. 11 isolates of actinomycetes produced indolyl-3-acetic acid (the level of synthesis was 21,0-62,5 μg/ml), 27 isolates produced siderophores and 6 isolates solubilized phosphorus compounds. Conclusions. Antimicrobial and plant growth promotional properties of the actinomycetes from rhizosphere D. antarctica were evaluated. Phytopathogenic bacteria and fungi antagonists were identified. A number of isolates were characterized by plant growth promotional properties that are combined with the synthesis of antimicrobial compounds. Such properties of isolated actinomycetes may play an important role in the adaptation of Antarctic plants to extreme conditions of existence. The described actinomycetes can be a source of new biologically active compounds and genes that control their biosynthesis.

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“…The pigment was extracted according to the method described previously [34] with some modifications. Briefly, 70 ml of C modified broth cultures were centrifuged at 9000 g for 10 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

et al. 2019

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Microorganisms thrive in extreme environments and are known for synthesizing valuable metabolites. Salt-loving microorganisms can flourish in saline environments which inhibit the growth of other microbial life, and they possess the potential to produce stable and novel biomolecules for the use in biotechnological applications, including anticancer compounds. Sambhar Lake is the largest inland soda lake in India and is an appropriate habitat for halophilic bacterial and archaeal strains in terms of diversity and potential production of bioactive compounds. In the present study, a moderately halo-alkaliphilic bacterial strain C12A1 was isolated from Sambhar Lake, located in Rajasthan, India. C12A1 was gram-positive, motile, rod-shaped, formed oval endospores, produced carotenoids, and exhibited optimal growth at 37 °C in 10–15% NaCl (pH 8). C12A1 was found to be able to hydrolyze skimmed milk, gelatin, and Tween 80 but unable to hydrolyze starch and carboxymethylcellulose. C12A1 showed 98.87% and 98.50% identity in 16S rRNA gene sequence to P. halophilus and P. salipiscarius, respectively. Nevertheless, C12A1 was clustered within the clade consisting of P. salipiscarius strains, but it showed a distinct lineage. Thus, C12A1 was designated as Piscibacillus sp. Cell proliferation assay results showed that C12A1 broth extract (BEP) decreased cell viability in breast cancer MDA-MB-231 cells, which was confirmed by the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Induction of cell toxicity was visualized by microscopy. Reverse Transcriptase PCR (RT-PCR) analysis demonstrated that BEP inhibited the expression of proliferative B-cell lymphoma-extra large (Bcl-xL) and cell cycle marker Cyclin-dependent kinase 2 (CDK2) at transcript levels. Similarly, cell migration and colony formation along with mesenchymal marker vimentin and stem cell marker BMI transcripts were found to be inhibited when cells were treated with the BEP. The anti-breast cancer potential of C12A1 indicates that microorganisms inhabiting saline-alkaline habitats, with Piscibacillus sp. in particular, are a promising source for discovery of novel bioactive substances.

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Studies on Siderophore and Pigment Produced by an Adhered Bacterial Strain Halobacillus trueperi MXM-16 from the Mangrove Ecosystem of Goa, India

Cited by 24 publications

References 21 publications

Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis

Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis

Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica E. Desv. (Galindez Island, Antarctica)

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

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