Studies on single-substrate, enzyme-catalyzed reactions and analysis of transition state by microcalorimetry

Wang, Tianzhi; Liu, Yi; Li, Weiping; Wan, Hongwen; Yan, Yalan; Wu, Dingquan; Song-Sheng, Qu

doi:10.1016/s0040-6031(97)00249-9

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…It was hypothesized that the laccase/mediator combination formed radicals within the lignocellulosic matrix that may have formed covalent bonds, thus increasing wet tensile strength. In the present study, high‐κ kraft pulps were reacted with laccase and gallic acid (3,4,5‐trihydroxybenzoic acid), a compound shown to react at a faster rate with laccase (28) compared to 4‐hba with a low K m value (29). The effects of laccase and gallic acid on pulp properties, dry strength, and wet strength of unrefined and refined pulp fibers was evaluated.…”

Section: Introductionmentioning

confidence: 99%

Modification of High Lignin Content Kraft Pulps with Laccase to Improve Paper Strength Properties. 1. Laccase Treatment in the Presence of Gallic Acid

Chandra¹,

Lehtonen²,

Ragauskas³

2008

Biotechnol Progress

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Laccase was reacted with gallic acid in the presence of a high-kappa (91) kraft pulp. The result was a modified pulp with 34%, 20%, and 72% improvements in burst, tensile, and wet tensile strength compared to untreated control samples. Fully bleached pulps were not responsive to the laccase treatment, indicating lignin was the major target for the fiber modification. The results indicate that the strength increases were a combined effect of improvements of hydrogen bonding between fibers and creation of phenoxy radical cross-links within the sheet.

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Section: Introductionmentioning

confidence: 99%