Studies on sodium-potassium-activated adenosine triphosphatase

Bonting, S.L.; Caravaggio, Leo L.; Canady, Mel Rose

doi:10.1016/s0014-4835(64)80007-5

Cited by 96 publications

(38 citation statements)

References 18 publications

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“…The corneal epithelial Na π /K π ATPase/total ATPase ratio found in this study (6%) similarly matches literature values (5%) (Bonting et al 1961). Ten weeks of hyperglycemia was found to have no significant effect on ouabain-sensitive corneal epithelial Na π / K π ATPase activity.…”

Section: Discussion I) Oxygen Uptakesupporting

confidence: 89%

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

Herse¹,

Petchell²

1998

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To more fully investigate the effect of hyperglycemia on the aerobic metabolism of the corneal epithelium. Methods: Corneal epithelial oxygen uptake rates and adenosine triphosphatase (ATPase) activities were measured in alloxan-induced diabetic and control rabbits over a 10 week period. Results: A transient reduction in epithelial oxygen uptake rate was seen at week 1. A chronic 14% reduction in oxygen consumption occurred after 6 weeks of hyperglycemia. Epithelial ouabain-sensitive ATPase activity was unaffected by 10 weeks of hyperglycemia. Epithelial ouabain-insensitive ATPase activity decreased 14% after 10 weeks of hyperglycemia. Conclusions: Ten weeks of hyperglycemia in the alloxan induced diabetic rabbit was associated with a 14% decrease in corneal oxygen uptake, a 14% decrease in corneal epithelial ouabain-insensitive ATPase activity and no change in corneal epithelial ouabain-sensitive ATPase activity. The Crabtree effect may help explain some of the clinical signs seen in the diabetic cornea as well as explaining why diabetic patients can wear contact lenses with minimal clinical problems.

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Section: Discussion I) Oxygen Uptakesupporting

confidence: 89%

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

Herse¹,

Petchell²

1998

Acta Ophthalmologica Scandinavica

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“…The activity of total ATPases was expressed as mmoles of phosphorous liberated/h/mg protein. Bonting (1970). To 1 ml of Tris buffer, 0.2 ml of each of MgSO 4 , KC1, NaCl, and EDTA were added and equilibrated at 37 C for 10 min and the enzyme reaction was initiated by the addition of 0.1 ml of sample.…”

Section: Estimation Of Total Atpasesmentioning

confidence: 99%

Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats

Al‐Numair

Veeramani

Alsaif

et al. 2015

Pharmaceutical Biology

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Context: Kaempferol is a flavonoid found in many edible plants (e.g. tea, cabbage, beans, tomato, strawberries, and grapes) and in plants or botanical products commonly used in traditional medicine. Numerous preclinical studies have shown that kaempferol have a wide range of pharmacological activities, including antioxidant, anti-inflammatory, anticancer, cardioprotective, neuroprotective, and antidiabetic activities. Objective: The present study investigates the effect of kaempferol on membrane-bound ATPases in erythrocytes and in liver, kidney, and heart of streptozotocin (STZ)-induced diabetic rats. Materials and methods: Diabetes was induced into adult male albino rats of the Wistar strain, by intraperitoneal administration of STZ (40 mg/kg body weight (BW)). Kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) was administered orally once daily for 45 d to normal and STZinduced diabetic rats. The effects of kaempferol on membrane-bound ATPases (total ATPase, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase) activity in erythrocytes and in liver, kidney, and heart were determined. Results: In our study, diabetic rats had significantly (p50.05) decreased activities of total ATPases, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase in erythrocytes and tissues. Oral administration of kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) for a period of 45 d resulted in significant (p50.05) reversal of these enzymes' activities to near normal in erythrocytes and tissues when compared with diabetic control rats. Discussion and conclusion: Thus, obtained results indicate that administration of kaempferol has the potential to restore deranged activity of membrane-bound ATPases in STZ-induced diabetic rats. Further detailed investigation is necessary to discover kaempferol's action mechanism.

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“…Enzymic antioxidants (CAT) (Aebi 1974), superoxide dismutase (SOD) (Marklund and Marklund 1974), glutathione-Stransferase (GST) (Habig et al 1974), glutathione peroxidase (GPx) (Rotruck et al 1973), non-enzymic antioxidant (GSH) (Moron et al 1979), thiobarbituric acid reactive substances (TBARS) (Ohkawa et al 1979), membrane bound ATPase, Na + K + ATPases (Bonting 1970), Ca 2+ ATPases (Hjerten and Pan 1983) and Mg 2+ ATPases (Ohnishi et al 1982) were carried out in tissue homogenate. LPO is expressed as nanomol TBA reactants/g of tissue, GSH in nanomol GSH/g of tissue.…”

Section: Assessment Of Oxidative Stress Status and Atpase Activitiesmentioning

confidence: 99%

Fish oil rich in eicosapentaenoic acid protects against oxidative stress-related renal dysfunction induced by TCDD in Wistar rats

Palaniswamy

Vishwanadha

Singaravelu

2014

Cell Stress and Chaperones

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Humans are systemically exposed to persistent organic pollutants, of which 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has become a major environmental concern. Exposure to TCDD results in a wide variety of adverse health effects which is mediated by oxidative stress through CYP1A1 activation and arachidonic acid metabolites. Eicosapentaenoic acid (EPA) exhibits antioxidant property and competes with arachidonic acid in membrane phospholipids and produces anti-inflammatory EPA derivatives. Since both EPA and its derivatives have been reported to enhance the antioxidant mechanism, the present study aimed at studying whether EPA could offer protection against TCDDinduced oxidative stress and nephrotoxicity in Wistar rats. Estimation of kidney markers (serum urea and creatinine) and histopathological studies revealed that EPA treatment significantly reduced TCDD-induced renal damage. TCDDinduced oxidative damage was reflected in a significant increase in CYP1A1 activity and lipid peroxide levels with a concomitant decline in non-enzymic antioxidant (GSH) and various enzymic antioxidants such catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), and glutathione peroxidase (GPx). In addition, TCDD-induced oxidative stress also resulted in decline in Na + -K + and Mg 2+ATPases activities with increase in Ca 2+ ATPases activity. Oral treatment with EPA showed a significant cytoprotection against TCDD-induced renal oxidative stress by decreased CYP1A1 activity and enhanced antioxidant status. TCDD-induced alterations in ATPase enzyme activities were also prevented by EPA treatment. Our results show clear evidence that EPA ameliorates TCDD-induced oxidative stress and kidney damage; thus suggest the potential of EPA as an effective therapeutic agent against toxic effects mediated through redox imbalance.

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Studies on sodium-potassium-activated adenosine triphosphatase

Cited by 96 publications

References 18 publications

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats

Fish oil rich in eicosapentaenoic acid protects against oxidative stress-related renal dysfunction induced by TCDD in Wistar rats

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