Studies on the Bacteremia of Bacterial Endocarditis

Abstract

During the period of the study the technique for processing blood cultures did not change. Blood cultures were prepared by inoculating 5 ml of ve¬ nous blood into each of two bottles. One bottle contained 100 ml of beef-heart-infusion broth and the other contained 100 ml of beef-heart-infusion broth with 0.2% dextrose added. The broth was 5 to 6 cm deep (and will

Early Detection of Typhoid by Polymerase Chain Reaction

“…The most important factor is the very few numbers of bacteria needed to cause severe infection, which can be as low as 10/mL. 19,20 Hence, positive culture yields are very low and elude definitive diagnosis. Other limiting factors, beside the bacteriostatic effect of antibiotics (already administered before the culture sample is taken), may be the nature of culture medium employed, the time of blood collection, the host's immune response system, and the intracellular characteristics of Salmonella typhi.…”

Diagnosis and Management of Infective Endocarditis and Its Complications

“…37,38 Continuous bacteremia and a high frequency of positive blood cultures are typical of this infection. In a study of 206 patients with blood culture- 39 However, the intensity of the bacteremia may not be great; fewer than 50 colony-forming units per milliliter of blood were detected in the majority of patients. 39 Blood cultures are negative in Յ5% of patients with IE diagnosed by strict diagnostic criteria.…”

“…In a study of 206 patients with blood culture- 39 However, the intensity of the bacteremia may not be great; fewer than 50 colony-forming units per milliliter of blood were detected in the majority of patients. 39 Blood cultures are negative in Յ5% of patients with IE diagnosed by strict diagnostic criteria. 40,41 Failure to culture the organism in IE may result from inadequate microbiological techniques, infection with highly fastidious bacteria or nonbacterial microorganisms, or most importantly, from the administration of antimicrobial agents before blood cultures are obtained.…”

Post-surgical Infective Endocarditis Involving Chordae Tendinae: A Rare Presentation

“…Amongst causative bacteria, aerobic gram-positive and anaerobic bacteria account for 70 to 80%, including Staphylococcus aureus, Streptococcus viridans, Enterococcus, coagulase -negative Staphylococcus, etc [6,7]. In the present case, blood cultures were performed 4 times but no organism was isolated.…”