During the period of the study the technique for processing blood cultures did not change. Blood cultures were prepared by inoculating 5 ml of ve¬ nous blood into each of two bottles. One bottle contained 100 ml of beef-heart-infusion broth and the other contained 100 ml of beef-heart-infusion broth with 0.2% dextrose added. The broth was 5 to 6 cm deep (and will

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The isolation of a Leptotrichia buccalis-like bacterium from the blood of a patient with endocarditis associated with a prosthetic aortic valve is reported. The organism was isolated from different blood cultures. It was similar but not completely identical to Leptotrichia buccalis. The microbiological identification was confirmed independently by three laboratories, including two reference institutions. The case and the characteristics of the microorganism are reported.

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