Studies on the binding of vinpocetine to human serum albumin by molecular spectroscopy and modeling

Jiang, Hua; Chen, Rong Rong; Wang, Hong Cui; Pu, Han Lin

doi:10.1016/j.cclet.2012.02.003

Cited by 11 publications

(2 citation statements)

References 8 publications

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“…The peak of BSA was slightly red shifted and decreased, which showed that the microenvironment around BSA was changed due to the V 18 . There are two kinds of fluorescence quenching, including dynamic and static quenching [ 20 , 21 ]. Due to the results, the absorbance peak of BSA was reduced by V 18 , and the fluorescence quenching of V 18 to BSA was likely to be static quenching.…”

Section: Resultsmentioning

confidence: 99%

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Zhang

Guo

et al. 2017

Molecules

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Polyoxometalates (POMs) are inorganic clusters that possess potential anti-bacterial, anti-viral, and anti-tumor activities. Herein, the in vitro anti-proliferation activities of K12[V18O42(H2O)]∙6H2O (V18) have been investigated on the MCF-7 and MDA-MB-231 cell lines. The results indicated that V18 could inhibit the proliferation of MCF-7 (IC50, 11.95 μM at 48 h) in a dose-dependent manner compared to the positive control, 5-fluorouracil (5-Fu, p < 0.05). The anti-proliferation activity of V18 might be mediated by arrest of the MCF-7 cells in the G2/M phase and induction of apoptosis and necrosis. Moreover, V18 can effectively quench the fluorescence of ctDNA. The binding mode between them may be groove or outside stacking binding. V18 can also effectively quench the intrinsic fluorescence of bovine serum albumin (BSA) and human serum albumin (HSA) via static quenching, and changed the conformation of BSA and HSA.

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Section: Resultsmentioning

confidence: 99%

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Zhang

Guo

et al. 2017

Molecules

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“…However, the mechanism of interaction between VCM and protein is poorly understood. In a previous paper, we reported optical spectroscopic studies designed to characterize the interaction of vinpocetine (VPC) with HSA . VPC, a semi‐synthetic alkaloid derivative of VCM, is a cerebrovascular drug with a structure similar to that of VCM.…”

Section: Introductionmentioning

confidence: 99%

Studies on the interaction between vincamine and human serum albumin: a spectroscopic approach

Jiang

Chen

et al. 2013

Luminescence

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The interaction between vincamine (VCM) and human serum albumin (HSA) has been studied using a fluorescence quenching technique in combination with UV/vis absorption spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy and molecular modeling under conditions similar to human physiological conditions. VCM effectively quenched the intrinsic fluorescence of HSA via static quenching. The binding constants were calculated from the fluorescence data. Thermodynamic analysis by Van't Hoff equation revealed enthalpy change (ΔH) and entropy change (ΔS) were -4.57 kJ/mol and 76.26 J/mol/K, respectively, which indicated that the binding process was spontaneous and the hydrophobic interaction was the predominant force. The distance r between the donor (HSA) and acceptor (VCM) was obtained according to the Förster's theory of non-radiative energy transfer and found to be 4.41 nm. Metal ions, viz., Na(+), K(+), Li(+), Ni(2+), Ca(2+), Zn(2+) and Al(3+) were found to influence binding of the drug to protein. The 3D fluorescence, FT-IR and CD spectral results revealed changes in the secondary structure of the protein upon interaction with VCM. Furthermore, molecular modeling indicated that VCM could bind to the subdomain IIA (site I) of HSA.

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Molecular docking and density functional theory calculations of vinpocetine and teicoplanin aglycone chiral selector

Roslan

Mustafa

Maarof

et al. 2020

J Incl Phenom Macrocycl Chem

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Studies on the binding of vinpocetine to human serum albumin by molecular spectroscopy and modeling

Cited by 11 publications

References 8 publications

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

The Anti-Proliferation Activity and Mechanism of Action of K12[V18O42(H2O)]∙6H2O on Breast Cancer Cell Lines

Studies on the interaction between vincamine and human serum albumin: a spectroscopic approach

Molecular docking and density functional theory calculations of vinpocetine and teicoplanin aglycone chiral selector

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