Studies on the binding of bacteria to glycolipids Two species of Propionibacterium apparently recognize separate epitopes on lactose of lactosylceramide

Abstract

Two species of Propionibucterium were analysed regarding their binding to glycosphingolipids. Bacteria were labeled with rzsI and selective interaction with glycolipids on thin-layer chromatograms was revealed by autoradiography. The carbohydrate site in common for active molecular species appeared to be lactose. The two bacteria differed, however, in the overall binding pattern on the chromatogram, probably due to recognition of separate epitopes on lactose. P. freudenreichii bound only to lactosylceramide while P. granuloszon also recognized substituted lactosylceramide: Galal+ 3Gal/31 -r4Glc/7Cer, GlcNAcj?l-r3Ga431+4Glc&Cer and Gab?l~3GlcNAcj?l~3Gal/.?l+4Glc~er were active, but Galal +4Gal/?l -Xilcj?Cer was inactive. Also, there was an interesting dependence on ceramide structure in the case of lactosylceramide. P. freudenreichii bound to lactosylceramide with sphingosine and non-hydroxy fatty acids but not to species with sphingosine and 2-hydroxy fatty acids, phytosphingosine and non-hydroxy fatty acids or phytosphingosine and 2-hydroxy fatty acids. For P. grunufosum the situation was reversed. This may be explained by an inthtence of ceramide structure on the presentation of the two lactose epitopes at the assay surface. These results were supported by curves from the binding of labeled bacteria to glycolipids coated in microtiter wells and in part by binding to glycolipid-coated chicken erythrocytes.

Identification of Target Tissue Glycosphingolipid Receptors for Uropathogenic, F1C-fimbriated Escherichia coli and Its Role in Mucosal Inflammation

“…A large number of commensal as well as pathogenic bacteria preferentially bind to lactosylceramide with phytosphingosine and/or hydroxy fatty acids, while the same bacteria are unable to bind to galactosylceramide and glucosylceramide (35)(36)(37)(38). This binding deficiency is independent of phytosphingosine and/or hydroxy fatty acids in the ceramide portion of the receptors.…”

“…Similar preparations were also isolated from canine and human tissues. A binding assay of 35 S-labeled ARD6 to non-acid glycosphingolipids separated on thin-layer chromatogram is shown in Fig. 1.…”

“…Binding of 35 S-labeled bacteria to glycosphingolipids on thin-layer chromatograms was performed as previously reported (28). Dried chromatograms were dipped for 1 min in diethylether/n-hexane (1:5, by volume) containing 0.5% (w/v) polyisobutylmethacrylate (Aldrich, Milwaukee, WI).…”

Polysialic acid engineering: synthesis of polysialylated neoglycosphingolipids by using the polysialyltransferase from neuroinvasive Escherichia coli K1.

“…Thus, while the significance of our present findings with respect to naturally occurring polysialylated gangliosides awaits further study, it seems likely that such polysialylated GSLs may have important biological and pharmacological functions. This relates particularly to the number of GSLs that are developmentally regulated heterophile antigens that function as surface receptors for bacteria, viruses, and toxins (40)(41)(42). Some GSLs are also tumor-associated antigens that have been implicated in transmembrane siling and as mediators for cell-cell interactions (41)(42)(43)(44)(45).…”

Solid-Phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-Time PCR

“…Often, the specific classes are expressed in specific tissues that can be exploited to find organisms that will bind cells from specific tissues. In addition, the binding of bacteria and toxins to gangliosides has been documented in the literature (7,13,16,22). To exploit this paradigm, we used host receptors (isolated mixed gangliosides) to mimic pathogen attachment onto the host cell to capture and concentrate pathogens from various samples onto a solid surface.…”