Studies on the biochemical effects of glibenclamide on alloxan diabetic rabbits

Annamala, P T; Augusti, K. T.

doi:10.1007/bf01975099

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Further, level of glycogen, the primary intracellular storable form of glucose in various tissues is a direct reflection of insulin activity as insulin promotes intracellular glycogen deposition by stimulating glycogen synthase and inhibiting glycogen phosphorylase. The observed depletion of liver glycogen stores in diabetic mice is consistent with an earlier study (27), indicating that it could be due to the loss of glycogen-synthase activating system (28) and/or increased activity of glycogen phosphorylase (27). Since alloxan causes selective destruction of E-cells of the islets of langerhans resulting in a marked decrease of insulin levels, it is rational to believe that the glycogen level in liver of diabetic animals will decrease, as synthesis of glycogen depends on insulin for the influx of glucose.…”

Section: Discussionsupporting

confidence: 91%

Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark

Sharma

Garg

Paul

2010

Indian J Clin Biochem

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Section: Discussionsupporting

confidence: 91%

Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark

Sharma

Garg

Paul

2010

Indian J Clin Biochem

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“…Diabetes was experimentally induced with streptozotocin. The effect of streptozotocin on the glycemic state of different animal species has been published in several reports (Gunnarson, et al, 1974;Annamala & Augusti, 1980;Yamamoto et al, 1981;Uchigata et al, 1983;Okamoto, 1984;Al-Awadi et al, 1985;Noreen et al, 1988Noreen et al, , 1992El-Seifi et al, 1993 a, b;Rawi, 1995;Rawi et al, 1996;Abdel-Moneim et al, 1997, 1999Judiono et al, 2011;Suharyo et al, 2012;Giovana et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref

Ünlü

Ahmed

et al. 2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Biochemical points of view, the preclinical treatment with kefir or insulin of normal or diabetic male rats, beneficially highly alternates the cellular activities, histochemical and immunohistochemical materials components, and histological architecture. of the pancreas therefore Kefir and insulin may, to some extent, repair the pathological side effects of type 1 diabetes mellitus.The positive results of using kefir and insulin in treating the Pathogen effects of type 1 diabetes mellitus on the pancreas of male mice gave us a hope of possibility to obtain positive clinical applications/implications. Therefore, these positive beneficial results make us continue our work and complete the various pre-clinical trial and clinical experiments phases.

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“…Several reports have been published on the effect of streptozotocin on the glycemic state of different animal species. (Gunnarson et al 1974;Annamala and Augusti, 1980;Yamamoto et al, 1981;Uchigata et al, 1983;Okamoto 1984;Al-Awadi et al, 1985;Noreen et al, 1988Noreen et al, , 1992El-Seifi et al, 1993 a, b;Rawi 1995;Rawi et al, 1996;Abdel-Moneim et al, 1997, 1999Judiono et al, 2011;Suharyo et al, 2012;Giovana et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref

Ünlü

Ahmed

et al. 2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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This study was designed to investigate the protective role of kefir and insulin consumption on cellular activities, histochemical components, histological architecture, and integrity of the liver by measuring IL-6 and TNFα in high fat-fed STZ-induced diabetic male Wistar rats.From a cell biological and histochemical point of view, both kefir and insulin have beneficial various effects on cellular activities and the histochemical components such as DNA, RNA, total protein, collagen, polysaccharides, and phospholipids materials synthesis in the examined normal and diabetic hepatocytes of the male rats. Biochemically, kefir and insulin have various biochemical effects on the serum IL-6 and TNF-α levels.From a pathological point of view, we can say the beneficial various cell biological and histochemical effects of kefir, pathologically may make kefir, to some extent, completely repair all the pathological side effects of type1 diabetes on the hepatocytes in diabetic rats. Also, insulin repair some of the pathological side effects of diabetes on the hepatocytes.The positive results of using kefir and insulin in treating the pathogen effects of diabetes make it possible to obtain positive clinical applications/implications. Therefore, these positive results encourage us to continue working and complete the various long-term pre-clinical trials and clinical trials phases. Composition of HFD: Induction of Diabetes Mellitus:Diabetes mellitus was experimentally induced in male animals by streptozotocin, STZ was dissolved in cold 0.01 M citrate buffer, pH 4.5, and always prepared freshly for immediate use within 5 minutes. Rats were fasted for overnight to induce diabetes by intraperitoneal (IP) injection of streptozotocin (STZ) at the dose of 45 mg/kg body weight (Judiono et al., 2011;Suharyo et al., 2012;Giovana et al., 2014). The normal control group was given citrate buffer without STZ. The development of diabetes was confirmed after 48 hours -7 days of STZ injection. The animals with fasting blood glucose levels of more than 200 mg/dl were considered as diabetic and included in this study. For glucose assay, blood samples were collected from the tail-tip or tail-vein of the rats and measured using a glucometer. Animal Grouping:The experimental animals were divided into six groups 10 animals each, 3 nondiabetic, and 3 diabetic groups: Experiment I: Group 1: Control animals, (negative group) were fed a standard diet plus oral administration of distilled water at a dose of 0.7 ml/animal/day. Group 2: Animals were fed a standard diet and received oral administration of kefir (0.7 ml/animal/day). Group 3: Animals received a high-fat diet (HFD) and additionally oral administration of kefir (0.7 ml/animal/day). Experiment II: Group A: Diabetic group (positive group), was fed HFD and received oral administration of distilled water (0.7 ml/animal/day). Group B: Diabetic animals received HFD plus oral administration of kefir (0.7 ml/animal/day. Group C: Diabetic group, fed HFD, was subcutaneously injected insulin (0.76 UI/200 m...

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Studies on the biochemical effects of glibenclamide on alloxan diabetic rabbits

Cited by 6 publications

References 22 publications

Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark

Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

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