Studies on the Cellular Physiological Activity of Lonicera japonica·Glycyrrhiza uralensis·Lentinula edodes Complex Extracts

Abstract

With ultraviolet rays, pollution and external stimuli acting as oxidation stress on the skin in line with recent changes in the global environment, modern people are increasingly relying on the frequency and dependence of cosmetics with certain effects. In particular, the characteristics of the skin of the elderly are that inflammation tends to occur due to the deterioration of the barrier function of the skin. To minimize this, research and development of cosmetics using natural materials that do not stimulate the skin and have effective effects are continuously increasing. The purpose of this study was to analyze the physiological activity of the Lonicera japonica•Glycyrrhiza uralensis FISCH•Lentinula edodes (LGL) complex extracts to confirm its potential as a cosmetic material. Our method are cytotoxicity test of LGL complex extracts in the HaCaT and RAW264.7 cells. RAW 264.7 The effects of cell protection from inflammation were measure in cells to identify anti-inflammatory effects. Also, to identify antioxidant effects, DPPH radical scavenging activity test and analysis of the total phenol and total flavonoid contents., tyrosinase inhibitory activity, elastase inhibitory activity, measured cell survival rate by UV-B irradiation in HaCaT. The results of this study are as follows. In the cytotoxicity test, the LGL complex extracts showed stable cell viability. RAW 264.7 cells treated with the LGL complex extracts showed a superior concentration-dependent inhibitory effects on Nitric oxide (NO) synthesis than cells treated with Lipopolysaccharide (LPS). In the DPPH radical scavenging activity test, the LGL complex extracts showed a high scavenging activity at 200 μg/mL of 50% EtOH. In the anti-inflammatory test. Moreover, the results of the analysis on the antioxidant activity of the LGL complex extracts confirmed the phenolic and flavonoid contents. In addition, when UV-B was run on HaCaT cells and absorbance was measured, the cell protection effect was shown to increase dependent on concentrations in the LGL complex extracts DW and 50% EtOH extracts. The tyrosinase inhibitory activity was found to increase concentration-dependent activity in 50% EtOH and 100% EtOH extracts of LGL complex extracts, indicating that active inhibition was more effective than positive control arbutin. Elastase inhibitory activity is shown to increase concentration-dependent at LGL complex extracts DW, 50% EtOH. Based on these results, the LGL complex extracts were found to have excellent cellular physiological activity. Accordingly, basic data on the development of functional cosmetics are provided and systematic and continuous research on the safety of natural products is required.