Studies on the collagenases of Clostridium histolyticum

The Histotoxic Clostridial Infections of Man 12

“…2) ,3-Toxin. This agent has been the subject of numerous studies since 1947 when Jennison (487) first claimed that C. histolyticum filtrates contained a collagenase, for it is an unusual and most interesting enzyme (136,137,244,372,645,660,763,1026). It will be recalled that, although 25 to 35% of the protein of the body consists of collagen (762), no mammalian enzymes and extremely few enzymes from other sources are known which can attack this substance in the undenatured state.…”

Role of green tea polyphenols in the inhibition of collagenolytic activity by collagenase

“…It constitutes more than 90% of the total solid matrix of the skin and this protein is stabilized into leather during tanning processes. Native collagen is susceptible to attack only by collagenase at physiological pH, temperature and ionic strength [26,27]. Collagen molecule is susceptible to attack by other proteases only after initiation of cleavage of the triple helix by collagenase [28].…”

Degradation of human collagen isoforms by Clostridium collagenase and the effects of degradation products on cell migration

“…It is a metalloproteinase that is able to digest triple‐helical type I, II and III collagens into simple peptides under physiological conditions (1,2). Clostridium collagenase contains more than one collagenolytic enzyme in the crude preparation (3). Mandl et al .…”