Role of green tea polyphenols in the inhibition of collagenolytic activity by collagenase

Madhan, Balaraman; Krishnamoorthy, Gurumoorthy; Rao, Jonnalagadda Raghava; Nair, Balachandran Unni

doi:10.1016/j.ijbiomac.2006.11.013

Cited by 145 publications

(114 citation statements)

References 36 publications

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“…There are many hydroxyl groups present in the TA which can act as hydrogen bond donor/ acceptor with different side chain groups of amino acids of collagen namely, aspartic acid (Asp), asparagine (Asn), hydroxyproline (Hyp), lysine (Lys) and serine (Ser). Previous report has also indicated the interaction of collagen with green tea polyphenol 27 . In addition, vegetable tannins have a long history of use in stabilizing collagen in the leather industry and are known to strongly binding to collagens 28 .…”

Section: Collagen-ta Interactionsmentioning

confidence: 86%

“…We found that TA is a competitive mode of inhibition of ChC activity, with a K i of 20 µM against FALGPA degradation. Previously, we reported the kinetics of inhibition of ChC by green tea polyphenolics 27 . Based on the accepted hydrolysis scheme for FALGPA by ChC 16 , TA can interact with ChC at site of the free ChC to form ChC-TA complex.…”

Section: Chc Inhibition Kineticsmentioning

confidence: 99%

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Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

Krishnamoorthy

Sehgal

Mandal

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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Section: Collagen-ta Interactionsmentioning

confidence: 86%

Section: Chc Inhibition Kineticsmentioning

confidence: 99%

Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

Krishnamoorthy

Sehgal

Mandal

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Hydroxyl groups of polyphenol could interact with the backbone or other functional group side chain of collagenase. In addition, hydrophobic interaction between the benzene ring of polyphenol and collagenase could also result in the conformational changes leading to unfunctioned enzyme (Madhan et al 2007). Another mechanism involves the Zn ion active site on collagenase.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications

Pientaweeratch

Panapisal

Tansirikongkol

2016

Pharmaceutical Biology

163

109

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Context Phyllanthus emblica L. (Euphorbiaceae) (amla), Manilkara zapota L.P. Royen (Sapotaceae) (sapota) and silymarin are reported to contain antioxidant effects. However, information on other biological activities relating to the anti-aging properties is limited. Objective To compare in vitro antioxidants, anti-collagenase (MMP-1 and MMP-2) and anti-elastase properties as well as the phenolic and flavonoid contents of amla, sapota and silymarin as potential anti-aging ingredients. Materials and methods The ethanol amla and sapota fruit extracts were prepared by three cycles of maceration with 24 h duration each. The total phenolic (TPC) and flavonoid (TFC) contents were determined. The antioxidant capacity was evaluated by DPPH and ABTS assays. The effects of MMP-1, MMP-2 and elastase inhibitions were determined by using the EnzChek Õ assay kits (Molecular-Probes, Eugene, OR). Results Amla exhibited the highest in TPC (362.43 ± 11.2 mg GAE/g) while silymarin showed the highest in TFC (21.04 ± 0.67 mg QE/g). Results of antioxidant activity by DPPH and ABTS methods showed that amla possessed the most potent capacity with IC 50 values of 1.70 ± 0.07 and 4.45 ± 0.10 mg/mL, respectively. Highest inhibitions against MMP-1, MMP-2 and elastase were detected for sapota with IC 50 values of 89.61 ± 0.96, 86.47 ± 3.04 and 35.73 ± 0.61 mg/mL, respectively. Discussion and conclusion Test extracts offered anti-aging properties in different mechanisms. Amla showed the highest phenolic content and antioxidant property with moderate anticollagenase. Silymarin exhibited measurable flavonoid content with anti-elastase effect. Sapota showed the highest collagenase and elastase inhibitions with moderate antioxidant effect. Thus, extracts might be added as a mixture to gain the overall anti-aging effects.ARTICLE HISTORY

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“…By hydrogen bonding and hydrophobic interactions with the collagenase, EGCG might lead to conformational changes or to masking of the catalytic region of MMP-2 24,25 . EGCG can also inhibit the activation of MMP-8 26 , which in turn was shown to affect the remineralisation of artificially demineralised dentin 27 .…”

mentioning

confidence: 99%

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Magalhães

Wiegand

Ríos

et al. 2009

Journal of Dentistry

129

121

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OBJECTIVES: This in situ/ex vivo study aimed to analyse the impact of possible MMPinhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. METHODS: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test. RESULTS: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions. CONCLUSIONS: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. 2Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ ABSTRACT Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. Methods:Twelve volunteers took part in this crossover and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoridefree toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60 s with: 250 ppm F solution (SnF 2 /AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24 ® , 100% Camellia Sinensis leaf extract, catechin concentration:30±3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (µm). The data were analysed by two-way repeated m...

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Role of green tea polyphenols in the inhibition of collagenolytic activity by collagenase

Cited by 145 publications

References 36 publications

Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

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