Studies on the constituents of PhilippinePiper betle leaves

Rimando, Agnes M.; Han, Byung Hoon; Park, Jeong Hill; Cantoria, Magdalena C.

doi:10.1007/bf02857217

Cited by 37 publications

(19 citation statements)

References 3 publications

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“…Major constituents for each oil that were more than 1% are as listed. These findings were in concordance with those previously reported for P.betle [14][15][16][17], P.sarmentosum [18][19][20], and P.nigrum [21], E.caryophyllata [22,23] and C.zeylanicum [8,12].…”

Section: Resultssupporting

confidence: 83%

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Said¹

2013

TOPROCJ

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Abstract:The aims of the study were to determine the in vitro cytotoxicity and anti-inflammatory activities of common edible herbs and spices on cultured human gingival fibroblasts (HGFs). Piper betle L. (betel leaf), P.sarmentosum Roxb.(wild betel / kadok leaf), P.nigrum L. (black pepper seed), Eugenia caryophyllata L. (clove bud) and Cinnamomum zeylanicum Blume (cinnamon bark). Essential oils were extracted using steam distillation technique and analysed using gas chromatography (GC) and gas chromatography -mass spectrometry (GC-MS). The HGFs were exposed to essential oils at 5 -0.04 µg/mL in less than 1% dimethylsulfoxide and the number of viable cells was counted to assess cytotoxicity effect. Anti-inflammatory action was determined via the inhibitory action of Interleukin-6 (IL-6), a major proinflammatory cytokine in the periodontal tissue inflammation. Treatment of fibroblasts with essential oils resulted in > 70% cell viability. The oils from black pepper seed, clove bud and cinnamon bark showed dose-dependent inhibitory action on IL-6 on cultured bacterial Lipopolysaccharide (LPS)-induced human gingival fibroblasts. Of all the oils, cinnamon bark oil showed the most prominent action comparable to acetylsalicylic acid. Conclusion: Essential oils of selected herbs and spices retained compatibility with gingival fibroblasts in culture and showed inhibitory activity on IL-6 released by LPS-induced HGFs. These findings suggest therapeutic potential for application of assay in the management of periodontal disease.

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Section: Resultssupporting

confidence: 83%

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Said¹

2013

TOPROCJ

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“…The synthesized ZnO NPs showed the LC 50 and r 2 values against the P. humanus capitis (11.80 mg/L; 0.966; Kirthi et al 2011). Some earlier workers have reported phenolics like chavibetol (53.1%) and chavibetol acetate (15.5%) isolated by the capillary GC analysis of the major constituents of Philippine Piper betle oil (Rimando et al 1986), and counter-current chromatography was used to isolate chavibetol from the essential oil from the leaves of Pimenta pseudocaryophyllus (dos Santos et al 2009). The chemical composition of the leaf oil of P. betle collected at Masjid Tanah, Melaka, Malaysia, is reported to have chavibetol (69.0%), eugenyl acetate (8.3%), and chavicol (6.0%) as the major components (Jantan et al 1994).…”

Section: Discussionmentioning

confidence: 93%

Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis (Phthiraptera: Pediculidae)

Bagavan¹,

Rahuman²,

Kamaraj³

et al. 2011

Parasitol Res

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The head lice, Pediculus humanus capitis De Geer is an obligate ectoparasite of humans that causes pediculosis capitis, a nuisance for millions of people worldwide, with high prevalence in children. P. humanus capitis has been treated by methods that include the physical remotion of lice, various domestic treatments, and conventional insecticides. None of these methods render complete protection, and there is clear evidence for the evolution of resistance and cross-resistance to conventional insecticides. Non-toxic alternative options are hence needed for head lice treatment and/or prevention, and natural products from plants are good candidates for safer control agents that may provide good anti-lice activity. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. The present study carried out the pediculocidal activity using the hexane flower bud extract of Syzygium aromaticum (Myrtaceae) against P. humanus capitis examined by direct contact and fumigant toxicity (closed- and open-container methods) bioassay. The chemical composition of S. aromaticum flower bud hexane extract was analyzed by gas chromatography-mass spectrometry. The major chemical constituent (58.79%) of flower bud hexane extract S. aromaticum was identified as chavibetol (5-allyl-2-methoxyphenol) by comparison of mass spectral data and retention times. The hexane extract of S. aromaticum was subjected to gas chromatography analysis, and totally 47 compounds were detected, of which chavibetol was predominantly present. The other major constituents present in the hexane extract were eugenol acetate (phenol,2-methoxy-4-(2-propenyl)-,acetate (15.09%), caryophyllene-(I1) (2,6,10,10-tetramethyl bicyclo [7.2.0] undeca-1,6-diene (13.75%), caryophyllene oxide (3.04%), 2,6,6,9-tetramethyl-1,4,8-cycloundecatriene (1.67%), and copaene (1.33%). The filter paper contact bioassay study showed pronounced pediculicidal activity in the flower bud hexane extract of S. aromaticum. The toxic effect was determined for every five in an 80-min treatment. The result showed percent mortality of 40, 82, and 100 at 5, 10, and 20 min, and the median lethal time (LT(50)) value was 5.83 (0.5 mg/cm(2)); 28, 82, and 100 at 5, 10, and 30 min. (LT(50) = 6.54; 0.25 mg/cm(2)); and 13, 22, 42, 80, and 100 at 5, 10, 20, 40, and 80 min (LT(50) = 18.68; 0.125 mg/cm(2)), respectively. The vapor phase toxicity was tested at 0.25 mg/cm(2). There was a significant difference in pediculicidal activity of S. aromaticum extract against P. humanus capitis between closed- and open-container methods. Adult mortalities were determined for every five in 60 min (closed method) and for every ten in 180 min (open method). The closed method showed the percent mortality was 45, 88, and 100 at 5, 10, and 15 min (LT(50) = 5.39), respectively. In the open-container method, the percent mortality was observed 5, 20, 47, 84, and 100 at 10, 20, 60, 120, and 180 min (LT(50) = 47.91), respectively. The mortality was more effective in the closed contai...

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“…camphene, β-caryophyllene, 1,8-cineole, p-cymene, limonene, α-pinene and β-pinene. 15 The essential oil of P. betle leaves from southern India contain safrole (39.9%), eugenol (9.0%), allo-pyrocatechol monoacetate (8.5%) and terpinen-4-ol (6.3%) as the major constituents. 16 Sharma et al reported eugenol at 82.2% and 90.5% and methyl eugenol at 6.9% and 4.1%, respectively, as the major components in P. betle cultivars originating from Desi Bangla and Ramtek Bangla, India, with p-cymene, α-terpineol and terpinyl acetate as minor components.…”

Section: Discussionmentioning

confidence: 99%

Components from Sri Lankan Piper betle L. leaf oil and their analogues showing toxicity against the housefly, Musca domestica

Mohottalage

Tabacchi

Guérin

2006

Flavour & Fragrance J

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Studies on the constituents of PhilippinePiper betle leaves

Cited by 37 publications

References 3 publications

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis (Phthiraptera: Pediculidae)

Components from Sri Lankan Piper betle L. leaf oil and their analogues showing toxicity against the housefly, Musca domestica

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