Studies on the Destruction of Red Blood Cells. Iv

Effect on Red Cells of a Small Rise in Temperature: in Vitro Studies

“…Damage was, only observed at temperatures above 46-47" C. (Ham et af., 1948;Valente et ai., 1965). A reciprocal relationship was demonstrated between temperature and time of exposure, the same destruction being provoked by an elevation of temperature or a prolongation of the action at constant temperature (Ham et a!., 1948).…”

“…The very rapid clearance from the circulation of radioactivity and its accumulation in the kidneys after the infusion of heat-treated Fe-labelled red cells may indicate intravascular haemolysis, and in that case it presumably represents a combination of thermal damage and a mechanical action in the circulation. This mechanism is mentioned by Ham et al (1948).…”

“…Other mechanisms, especially the possibility of changes in the lipoprotein structure of the cell membranes, are discussed.In a single study, human red cells exhibited the same vulnerability as rabbit red cells to small elevations of temperature, although their sensitivity was less.The influence of temperature upon haemolytic mechanisms has been the subject of many investigations. The effect of temperature on osmotic fragility is well known, but has mostly been studied in the range 0-40" C. (Jacobs and Parpart, 1931;Parpart, Lorenz, Parpart, Gregg and Chase, 1947;Mortensen, 1963), whereas heat damage to red cells has usually been examined using temperatures above 45" C. (Ham, Shen, Fleming and Castle, 1948; Schubothe, 1960; Kimber and Lander, 1964; Valente, Gatti and Spinelli Ressi, 1965). Changes in the viability of red cells, due to heating over the biological range of temperature, has only had sporadic mention.During experimental fever in rabbits an increased destruction of erythrocytes has been demonstrated (Karle, 1968a) ; on this basis it was of interest to study in vitro the effect on red cells of elevating the temperature to the range seen in fever.…”

“…The influence of temperature upon haemolytic mechanisms has been the subject of many investigations. The effect of temperature on osmotic fragility is well known, but has mostly been studied in the range 0-40" C. (Jacobs and Parpart, 1931;Parpart, Lorenz, Parpart, Gregg and Chase, 1947;Mortensen, 1963), whereas heat damage to red cells has usually been examined using temperatures above 45" C. (Ham, Shen, Fleming and Castle, 1948; Schubothe, 1960; Kimber and Lander, 1964; Valente, Gatti and Spinelli Ressi, 1965). Changes in the viability of red cells, due to heating over the biological range of temperature, has only had sporadic mention.…”

Consequences Arising From Elevated Surface Temperatures on Human Blood

“…Several in vitro investigations by other researchers addressed the influence of elevated temperatures on blood and its constituents (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). It was reported that thermally induced hemolysis of human erythrocytes occurs when they are exposed to elevated temperatures above normal body temperature (4-7), with the initiation of hemolysis to be a function of temperature and time.…”