Studies on the Enamel Structure of Transplanted Tooth Germ

Abstract

Enamel covers the dentin in mammalian teeth. It is believed that the enamel structure reflects the function of teeth, since the enamel structure is different depending on an animal's feeding habits. Tooth germ transplantation is a popular method for developmental and tissue engineering research. However, there is little information about the structure of the enamel of transplanted tooth germ, although tooth structure, especially enamel structure, is very important for the function of teeth. In this study, the enamel structure of E13.5 mouse mandible first molars transplanted into the kidney capsule was observed by scanning electron microscopy. Many part of the tooth crown are formed at transplantation 7 day. Although enamel and dentin were observed at the cusp portion, hard tissue was not formed at the cervical portion. The enamel prisms were observed at day 21 after transplantation. The enamel was divided into three layers according to the running pattern of the enamel prisms as normal tooth germ in vivo. However, the width of the various layers was different. It seems likely that the microenvironment surrounding tooth germ may play an important role in determining the structure of the enamel, since normal tooth germ grows in the calcified alveolar bone according as development.

Efficient induction of functional ameloblasts from human keratinocyte stem cells

“…The appearance of prisms is determined by the orientation of the crystals. According to the study of enamel structure in human molars, the enamel is divided into three layers by the running pattern of the enamel prisms [ 43 , 44 ]. In this work, similar images of the running pattern of the enamel prisms were observed for the human molar and the human–mouse chimeric teeth cultured for 20 or 60 days, indicating that the enamel prism microstructure of the human–mouse chimeric teeth is likely to grow completely after cultivation for more than 20 days.…”