Studies on the growth and survival of<i>Staphylococcus aureus</i>in corned beef

Mansfield, John M.; Farkas, G. L.; Wieneke, Antonnette A.; Gilbert, R. J.

doi:10.1017/s0022172400060514

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…(3) In 1979 more than 85 people in 9 incidents became ill over a 5-month period after eating corned beef from freshly opened 6 lb cans all from the same production plant in Brazil [26]. S. aureus was isolated from all incidents and four of the isolates produced SEA and were lysed by phage 29.…”

Section: Examples Of Outbreaksmentioning

confidence: 99%

Staphylococcal food poisoning in the United Kingdom, 1969–90

1993

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SUMMARYBetween 1969 and 1990 strains ofStaphylococcus aureusfrom 359 outbreaks and sporadic cases of staphylococcal food poisoning in the United Kingdom were examined in the PHLS Food Hygiene Laboratory for the production of enterotoxin. In a number of instances the incriminated foods were also examined for the presence of enterotoxin. Strains from 79% of incidents produced enterotoxin A alone or together with another enterotoxin. The level ofS. aureuspresent in the foods ranged from no viableS. aureusdetected to 1.5 × 1010c.f.u./g with a median of 3.0 × 107c.f.u./g. Enterotoxin was detected in foods in the absence of viableS. aureusin only two outbreaks and in both cheese was the implicated food. Meat. poultry or their products were the vehicle in 75% of incidents with ham and chicken most frequently implicated. Other foods included fish and shellfish (7%) and milk and milk products (8%). Most contamination took place in the home followed by restaurants and shops. Seventy-one percent of the incident strains were lysed by phages of group III or I/III.

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Section: Examples Of Outbreaksmentioning

confidence: 99%

Staphylococcal food poisoning in the United Kingdom, 1969–90

1993

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“…Constituents of the original medium, included to assist repair of staphylococci exposed to adverse intrinsic and extrinsic factors (Baird-Parker & Davenport 1965; Collins-Thompson et al. 1974;Gray et al 1974;Idziak & Mossel 1980), proved to be ineffective in some situations (Mansfield et al 1983;Isigidi et al 1989). Procedures based on solid medium repair (SMR) (Fig.…”

Section: S E L E C T I V E E N U M E R a T I O N Of Staphylococcus Aumentioning

confidence: 99%

Staphylococcus aureus and related staphylococci in foods: ecology, proliferation, toxinogenesis, control and monitoring

Mossel¹,

Netten

1990

Journal of Applied Bacteriology

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“…It successfully survives in high saline up to 25% NaCl, which is commonly found in canned foods. The low water activity (a w ) makes the bacteria uniquely resistant to drying and capable of growing and producing enterotoxins in foods with low a w ( 7 , 8 ). S. aureus lives in highly alkaline (pH up to 9.5) conditions that are found in garden soil, sewage, ground water, and some parts of the human gut ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Roles of Staphylococcus aureus Mnh1 and Mnh2 Antiporters in Salt Tolerance, Alkali Tolerance, and Pathogenesis

et al. 2018

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Staphylococcus aureus has three types of cation/proton antiporters. The type 3 family includes two multisubunit Na+/H+ (Mnh) antiporters, Mnh1 and Mnh2. These antiporters are clusters of seven hydrophobic membrane-bound protein subunits. Mnh antiporters play important roles in maintaining cytoplasmic pH in prokaryotes, enabling their survival under extreme environmental stress. In this study, we investigated the physiological roles and catalytic properties of Mnh1 and Mnh2 in S. aureus. Both Mnh1 and Mnh2 were cloned separately into a pGEM3Z+ vector in the antiporter-deficient KNabc Escherichia coli strain. The catalytic properties of the antiporters were measured in everted (inside out) vesicles. The Mnh1 antiporter exhibited a significant exchange of Na+/H+ cations at pH 7.5. Mnh2 showed a significant exchange of both Na+/H+ and K+/H+ cations, especially at pH 8.5. Under elevated salt conditions, deletion of the mnhA1 gene resulted in a significant reduction in the growth rate of S. aureus in the range of pH 7.5 to 9. Deletion of mnhA2 had similar effects but mainly in the range of pH 8.5 to 9.5. Double deletion of mnhA1 and mnhA2 led to a severe reduction in the S. aureus growth rate mainly at pH values above 8.5. The effects of functional losses of both antiporters in S. aureus were also assessed via their support of virulence in a mouse in vivo infection model. Deletion of the mnhA1 gene led to a major loss of S. aureus virulence in mice, while deletion of mnh2 led to no change in virulence.IMPORTANCE This study focuses on the catalytic properties and physiological roles of Mnh1 and Mnh2 cation/proton antiporters in S. aureus and their contributions under different stress conditions. The Mnh1 antiporter was found to have catalytic activity for Na+/H+ antiport, and it plays a significant role in maintaining halotolerance at pH 7.5 while the Mnh2 antiporter has catalytic antiporter activities for Na+/H+ and K+/H+ that have roles in both osmotolerance and halotolerance in S. aureus. Study of S. aureus with a single deletion of either mnhA1 or mnhA2 was assessed in an infection model of mice. The result shows that mnhA1, but not mnhA2, plays a major role in S. aureus virulence.

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Studies on the growth and survival ofStaphylococcus aureusin corned beef

Cited by 8 publications

References 13 publications

Staphylococcal food poisoning in the United Kingdom, 1969–90

Staphylococcal food poisoning in the United Kingdom, 1969–90

Staphylococcus aureus and related staphylococci in foods: ecology, proliferation, toxinogenesis, control and monitoring

Roles of Staphylococcus aureus Mnh1 and Mnh2 Antiporters in Salt Tolerance, Alkali Tolerance, and Pathogenesis

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