(Studies on the gynogenesis in Hirame Paralichthys olivaceus. VII). Induction of gynogenetic diploids in Paralichthys olivaveus by suppression of the 1st cleavage with special reference to their survival and growth.

Tabata, Kimiyo; Gorie, Shigeaki

doi:10.2331/suisan.54.1867

Cited by 20 publications

(13 citation statements)

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“…However, no heterozygosity was observed at these loci in mitotic‐G2N. These results suggested that the production of gynogenetic diploids was due to suppression of first cleavage and homozygosity of mitotic‐G2N (Tabata and Gorie 1988; Taniguchi et al. 1988).…”

Section: Discussionmentioning

confidence: 85%

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Kato

Murata

Yamamoto

et al. 2008

Journal of Applied Ichthyology

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Two types of gynogenetic diploids were arti®cially induced in the red sea bream (Pagrus major Temminck et Schlegel), either by suppressing the ®rst cell cleavage (mitotic-G2N) or by retaining the second polar body (meiotic-G2N). The eggs of red sea bream were inseminated with UV-irradiated (3000 erg mm )2 ) sperm of Japanese parrot ®sh (Oplegnathus fasciatus Temminck et Schlegel), and hydrostatic pressure shock of 700 kg cm )2 for 5.5 min at 46 min after insemination (mitotic-G2N) and cold shock of 1°C for 30 min at 3 min after insemination (meiotic-G2N) were applied to the eggs, sequentially. The total hatching rate and hatching rate of normal larvae of the normal diploid, meiotic-G2N and mitotic-G2N were 86.5 and 94.9%, 38.1 and 45.8%, and 12.8 and 35.0%, respectively. The induction of mitotic-G2N was con®rmed by isozyme marker analysis. The standard deviations, variances and coecients of variation of the body weight, standard length and body depth in 91-day-old juveniles were always large in mitotic-G2N, small in normal-2N and intermediate in meiotic-G2N. The variances in the number of pectoral ®n rays and caudal ®n rays of mitotic-G2N were signi®cantly higher than those of normal-2N. The incidences of deformities were highest in the mitotic-G2N group. The survival rates and growth performance of the meiotic-and mitotic-G2N were signi®cantly lower than those of normal-2N. Both G2N survived for 3 years to the adult stage.

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Section: Discussionmentioning

confidence: 85%

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Kato

Murata

Yamamoto

et al. 2008

Journal of Applied Ichthyology

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“…However, this was different from the UV doses in this species reported by Japanese researchers. Tabata et al (1986) reported UV irradiation doses from 660 to 11,000 erg mm -2 , while the practical intensity of Tabata and Gorie (1988) and Yamamoto (1999) reports were 4,800 and 4,560 erg mm -2 , respectively. These doses seem too low in view of our results, which could be explained by the different depths of sperm during UV irradiation used in these studies.…”

Section: Discussionmentioning

confidence: 95%

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

You

Sun

et al. 2008

Aquacult Int

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Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig's effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm -2 . At 15°C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3°C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1°C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UVirradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring.

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“…4,20,24) To identify the type of gynogenesis, marker isozyme genes with nearly 100% gene-centromere (G-C) recombination rate were used. 2,16,17,19,20,21,25) In the loath, MPI* had a very high rate of G-C recombination and appear to be a convenient gene marker for verifying the type of gynogenesis. About 96% of the progeny of the meiotic gynogens were heterozygous at the MPI* locus, while the specimens randomly chosen from the groups of mitotic gynogens from the same female revealed only homozygous genotypes.…”

Section: Discussionmentioning

confidence: 99%

Suppression of the First Cleavage and Cytogenetic Studies on the Gynogenetic Loach

1994

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Optimum conditions of hydrostatic pressure were examined for induction of two types of gynogenetic diploids (meiotic and mitotic) in the loath. Hydrostatic pressure treatment of 700 and 800k g/cm2 for 1 min duration gave the best yield of normal fry when the treatment began 5 min after fertilization by inhibiting the second meiosis of fertilized eggs. For mitotic gynogenesis by suppression of the first cleavage, the optimum time of application of the pressure (800 kg/cm2, 1 min duration) was determined to be 30 or 35 min after fertilization.In the early stage of embryos of mitotic gynogens, various aneuploids with cytogenetic aberrations were detected, in addition to induced diploids and miss-induced haploids. These were considered the major factors responsible for poor development and survival. All hatched fry of the mitotic gynogens were determined as diploid or near-diploid even in the case of severely deformed fry.Homozygosity of the mitotic gynogens was confirmed by isozyme analysis at the protein locus MPI* which has a very high rate of gene-centromere recombination.

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(Studies on the gynogenesis in Hirame Paralichthys olivaceus. VII). Induction of gynogenetic diploids in Paralichthys olivaveus by suppression of the 1st cleavage with special reference to their survival and growth.

Cited by 20 publications

References 0 publications

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

Suppression of the First Cleavage and Cytogenetic Studies on the Gynogenetic Loach

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