Studies on the interaction between rutin and DNA in the absence and presence of β‐cyclodextrin by electrochemical and spectroscopic methods

Abstract

bThe interactions between rutin or the inclusion complex of rutin-Fcyclodextrin and DNA were investigated by means of cyclic voltammetry, W-vis absorption spectroscopy and fluorescence emission spec~oscopy. The apparent binding constant of rutin with DNA is found to be 2.9X lo4 LJmol. The results showed that the benzopyranic-4-one plane of rutin mainly intercalated into DNA in the absence of pcyclodextrin, while the catecholic portion of rutin was located in the double helix of DNA in the presence of pcyclodextrin.

Studies of the interactions of 4-carboxyl-2,6-dinitrophenylazohydroxynaphthalenes with CT-DNA in aqueous medium

“…For small molecules binding to active sites on a macromolecule, the formation or binding constant can be estimated from the expression in Eq. (1) [28][29][30][31][32][33].…”

“…The straight trend obtained is the typical Benesi-Hildebrand plot for the estimation of the formation constant of molecular complexes [28][29][30][31][32][33]. From the plot of A 0 /(A − A 0 ) against 1/[DNA], the ratio of the intercept to the slope gives the formation constant, K f .…”

Synthesis, characterization and DNA binding studies of penta- and hexa-coordinated diorganotin(IV) 4-(4-nitrophenyl)piperazine-1-carbodithioates

“…6B shows that the peak position of 30 lM 2 is shifted bathochromically from k max = 396 to 403 nm by the addition of 80 lM DNA, accompanied with hypochromic shift from 0.796 to 0.307. These remarkable spectral characteristics suggest intercalation of 2 into the stacked base pairs domain of DNA [31][32][33]. The hypochromic effect is caused by the overlapping of the electronic states of the intercalating chromophore of the complex 2 with the DNA bases [34] and the bathochromic shift is caused by the lowering in pp * transition energy of the complex due to its ordered stacking between the DNA base pairs after intercalation [35].…”

Electrochemistry of Cyclodextrin Inclusion Complexes of Pharmaceutical Compounds

“…The interactions between rutin or the inclusion complex of rutin--cyclodextrin and DNA were investigated by cyclic voltammetry [65]. The results showed that the benzopyranic-4-one plane of rutin mainly intercalated into DNA in the absence of -cyclodextrin, while the catecholic portion of rutin was located in the double helix of DNA in the presence of -cyclodextrin.…”