Studies on the Lipoprotein Lipases of Microorganisms

Abstract

The production of lipoprotein lipases by Pseudomonas sp. M-12-33 was improved by an increased aeration. Glucose stimulated the growth of the bacterium but reduced the enzyme production in Bouillon medium. Olive oil remarkably increased the production of lipoprotein lipases but the effect was cancelled by calcium carbonate. Stearic and palmitic acids were as effective as olive oil, but capric and caprylic acids inhibited the enzyme production. Urea was proved to be very effective as '" nitrogen source for the production of lipoprotein lipases.

Studies on sterol metabolism by microorganisms. IV. Production of cholesterol esterase by Pseudomonas fluorescens.

“…It seems likely that the lipid plays an essential role in the formation of cholesterol esterase by P. fluorescens. A similar lipid require ment for enzyme production has been observed with lipoprotein lipase of Pseudomonas sp., 22) lipases of Candida paralipolytica23) and Geotri chum candidum Link. 24) The time course of the culture of Pseudo monas fluorescens showed that the enzyme for mation in the culture fluid was observed in an early stage of growth when cells were not prone to lysis.…”

Production of Cholesterol Esterase byPseudomonas fluorescens

“…It seems likely that the lipid plays an essential role in the formation of cholesterol esterase by P. fluorescens. A similar lipid requirement for enzyme production has been observed with lipoprotein lipase of Pseudomonas Sp., 22) lipases of Candida paralipolytica 23 ) and Geotrichum candidum Link.24J…”