Studies on the Male Antifertility Agent Gossypol Acetic Acid VII. Effect of Motility Stimulated Factors on the Revival of Human Spermatozoal Motility after Gossypol Treatment in vitro

Abstract

Human spermatozoa were incubated with gossypol acetic acid (100 pg/ 1 x lo6 spermatozoa/ml) at 37" C for 30 min. The drug treatment inhibited the spermatozoal motility significantly. Washing of the spermatozoa, after gossypol treatment, did not effect their motility. A partial revival in the motility of the spermatozoa was observed when gossypol treated spermatozoa were incubated, after washing, with motility stimulating factors, e.g. theophylline, dibutyrylcAMP and Kallikrein.

Structure, properties of gossypol and its derivatives—from physiological activities to drug discovery and drug design

“…However, gossypol acetic acid 40 significantly inhibited the spermatozoal motility. 160 Loberg et al 161 established a prostate cancer model with prostate cancer cells VCaP. (−)-Gossypol acetic acid 40 in combination with surgical castration delayed the onset of androgen-dependent prostate cancer by disrupting the antiapoptotic activity of Bcl-2 proteins.…”

Silica Gel Chromatographic Methods for Identification, Isolation and Purification of Gossypol Acetic Acids Prepared in Lab

“…It is usually prepared as gossypol acetic acid (GAA). GAA shows a raising interest because of its biological properties such as: anticancer [1], antiviral [2,3], antimicrobial and antifertility properties [4][5][6]. It can inhibit cellular macromolecules [7].…”