Studies on the morphological changes in the midguts of two ixodid tick species Boophilus microplus and Rhipicephalus appendiculatus during digestion of the blood meal

Agyei, A. D.; Runham, N. W.

doi:10.1016/0020-7519(94)00114-4

Cited by 55 publications

(48 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tick blood digestion pattern supported by previous ultramorphological observation of lysosome in midgut digestive cells consists of three distinct phases: phase-1, slow feeding process but rapid digestion activity in midgut digestive cells; phase-2, rapid feeding process but slow digestion in the cells, which starts at 12-36 hr before engorgement; and phase-3, continuous digestion phase during post-engorgement period [2,18,28,32]. Consistently, midgut HlLAP mRNA expression was quite synchronized with the tick blood digestion pattern.…”

Section: Discussionsupporting

confidence: 55%

Leucine Aminopeptidase in the Ixodid Tick Haemaphysalis longicornis: Endogenous Expression Profiles in Midgut

et al. 2009

View full text Add to dashboard Cite

ABSTRACT. We previously identified a cDNA from the ixodid tick Haemaphysalis longicornis that encodes leucine aminopeptidase, HlLAP. Functionally, recombinant HlLAP effectively hydrolyzed synthetic amino acid derivatives. Here, we investigated the temporal expression profiles of midgut HlLAP in adult H. longicornis parthenogenetic ticks from the starting of blood feeding until just before the onset of oviposition. Midgut HlLAP transcript expression level was higher during post-engorgement period than that during feeding period. Endogenous HlLAP in the midgut was also observed with higher expression level during post-engorgement period. Histological localization of HlLAP was in the cytosol of midgut epithelial cells, notably the newly differentiated basophilic cells at post-engorgement. Our data suggested that HlLAP was dominantly localized in basophilic cells, where it may play regulatory roles in protein biosynthesis and degradation.

show abstract

Section: Discussionsupporting

confidence: 55%

Leucine Aminopeptidase in the Ixodid Tick Haemaphysalis longicornis: Endogenous Expression Profiles in Midgut

et al. 2009

View full text Add to dashboard Cite

show abstract

“…At the same time it seems to be an alteration that is reversible by blood intake as shown by ticks of dogs fed for 96 and 120 h and which lacked such microscopic aspect. This process is probably related to the shedding of empty digest cells from unfed ticks followed by a massive proliferation and differentiation of stem cells in the engorging ticks as described by Agyei and Runham (1995) for Boophilus microplus and Rhipicephalus appendiculatus. On the other hand it is noteworthy that vacuolization of midgut cells was also observed in 31.8 and 52.4% of ticks fed on guinea pigs during first and third infestation, respectively.…”

Section: Discussionmentioning

confidence: 95%

Histopathology of Rhipicephalus sanguineus (Acari: Ixodidae) ticks fed on resistant hosts

et al. 2009

View full text Add to dashboard Cite

Histological features of Rhipicephalus sanguineus ticks fed on dog, a non resistant host, and on guinea pig, a resistant host, were compared. Unfed ticks and ticks from each host species were collected during first and third infestation and processed for histology. Many ticks from guinea pigs, especially during third infestation, were unattached, dehydrated and small. Only the midgut of ticks fed on guinea pigs had host leukocytes. Vacuolization of midgut cells was observed in all ticks, with exception of those fed on dogs for more than 96 h. Ticks of guinea pigs, particularly from third infestation, had vacuolated tracheae and swelling of malpighian tubules. Solely ticks from third infestation of guinea pigs displayed vacuolization of oocytes. Ticks fed on guinea pigs also had an increased number of guanine spherules. Observed alterations in ticks from guinea pigs are discussed.

show abstract

“…On the first day after dropping, the epithelium showed two distinct cellular types bound to the basal membrane, that we named basophilic cells and digest cells, in agreement with previous descriptions (Agbede and Kemp, 1985) (Fig.·1A,B). Basophilic cells have been categorised by some authors as stem cells that generate digest cells (Agyei and Runham, 1995), whereas others describe basophilic cells as a differentiated cell lineage (Agbede and Kemp, 1985). The basophilic cells were caliciform in shape on the first and third days, but had changed to a round shape by day 15 (Fig.·1C) and were finally flattened by day 20 (Fig.·1D).…”

Section: Resultsmentioning

confidence: 99%

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Lara

Lins

Paiva-Silva

et al. 2003

Journal of Experimental Biology

107

View full text Add to dashboard Cite

The hard tick Boophilus microplus ingests large volumes of cattle blood, as much as 100 times its own mass before feeding. Huge amounts of haem are produced during haemoglobin digestion, which takes place inside acidic lysosomal-type vacuoles of the digest cells of the midgut. Haem is a promoter of free radical formation, so haemoglobin digestion poses an intense oxidative challenge to this animal.In the present study we followed the fate of the haem derived from haemoglobin hydrolysis in the digest cells of the midgut of fully engorged tick females. The tick does not synthesize haem, so during the initial phase of blood digestion, absorption is the major route taken by the haem, which is transferred from the digest cells to the tick haemocoel. After this absorptive period of a few days, most of the haem produced upon haemoglobin degradation is accumulated in the interior of a specialized, membrane-delimited, organelle of the digest cell, herein called hemosome. Haem accounts for 90% of the hemosome mass and is concentrated in the core of this structure, appearing as a compact, non-crystalline aggregate of iron protoporphyrin IX without covalent modifications. The unusual FTIR spectrum of this aggregate suggests that lateral propionate chains are involved in the association of haem molecules with other components of the hemosome, which it is proposed is a major haem detoxification mechanism in this bloodsucking arthropod.

show abstract

Studies on the morphological changes in the midguts of two ixodid tick species Boophilus microplus and Rhipicephalus appendiculatus during digestion of the blood meal

Cited by 55 publications

References 16 publications

Leucine Aminopeptidase in the Ixodid Tick Haemaphysalis longicornis: Endogenous Expression Profiles in Midgut

Leucine Aminopeptidase in the Ixodid Tick Haemaphysalis longicornis: Endogenous Expression Profiles in Midgut

Histopathology of Rhipicephalus sanguineus (Acari: Ixodidae) ticks fed on resistant hosts

A new intracellular pathway of haem detoxification in the midgut of the cattle tick Boophilus microplus: aggregation inside a specialized organelle, the hemosome

Contact Info

Product

Resources

About