Studies on the Opioid Mechanism in Tetrahymena

Renaud, Fernando L.; Chiesa, Ricardo; Rodrı́guez, Fernando I.; Tomassini, Nilka; Marino, Michael J.

doi:10.1007/978-3-642-80106-8_2

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…Its rate of phagocytosis shows a pharmacologically well‐characterized inhibition by morphine and endogenous mammalian opioids. The Tetrahymena response to these opioids has striking pharmacological similarity to that of the mouse macrophage (Renaud et al 1996). EST 213 appears to be related to a Chlamydomonas intraflagellar transport gene and its functional analysis is underway (Brown, Fine, Pearlman & Gaertig, pers.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila

Fillingham

Chilcoat

Turkewitz

et al. 2002

J Eukaryotic Microbiology

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To assess the utility of expressed sequence tag (EST) sequencing as a method of gene discovery in the ciliated protozoan Tetrahymena thermophila, we have sequenced either the 5' or 3' ends of 157 clones chosen at random from two cDNA libraries constructed from the mRNA of vegetatively growing cultures. Of 116 total non-redundant clones, 8.6% represented genes previously cloned in Tetrahymena. Fifty-two percent had significant identity to genes from other organisms represented in GenBank, of which 92% matched human proteins. Intriguing matches include an opioid-regulated protein, a glutamate-binding protein for an NMDA-receptor, and a stem-cell maintenance protein. Eleven-percent of the non-Tetrahymena specific matches were to genes present in humans and other mammals but not found in other model unicellular eukaryotes, including the completely sequenced Saccharomyces cerevisiae. Our data reinforce the fact that Tetrahymena is an excellent unicellular model system for studying many aspects of animal biology and is poised to become an important model system for genome-scale gene discovery and functional analysis.

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Section: Discussionmentioning

confidence: 99%

Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila

Fillingham

Chilcoat

Turkewitz

et al. 2002

J Eukaryotic Microbiology

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LOCALIZATION OF β‐ENDORPHIN IN TETRAHYMENA BY CONFOCAL MICROSCOPY. INDUCTION OF THE PROLONGED PRODUCTION OF THE HORMONE BY HORMONAL IMPRINTING

Csaba

Kovács

1999

Cell Biology International

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The unicellular Tetrahymena has hormone receptors and hormones which are characteristic of higher vertebrates, as well as similar signal transduction pathways. In the present experiments, immunocytochemistry and confocal microscopy were used to study the presence and localization of beta-endorphin in Tetrahymena pyriformis GL. Endorphin (or endorphin-like material) was localized in the cortical structures, oral field, cilia and nuclear envelope. One-hour treatment with beta-endorphin ('hormonal imprinting') increased the presence of immunocytochemically demonstrable endorphin immediately and after 24 h, and was especially strong after 96 h of treatment. Simultaneous treatment with naloxone, an opioid antagonist, did not inhibit endorphin effect, but had an additive effect on endorphin production. Naloxone alone induced a very intensive accumulation of endorphin 96 h after treatment. The results support the possibility of a hormone production being induced by the imprinting procedure, but the imprinter-like effect of naloxone also points to the importance in this case of non-discriminatory receptors also being involved in the process.

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Hormonal Communication of Tetrahymena

Csaba

2016

Biocommunication of Ciliates

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Studies on the Opioid Mechanism in Tetrahymena

Cited by 6 publications

References 21 publications

Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila

Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila

LOCALIZATION OF β‐ENDORPHIN IN TETRAHYMENA BY CONFOCAL MICROSCOPY. INDUCTION OF THE PROLONGED PRODUCTION OF THE HORMONE BY HORMONAL IMPRINTING

Hormonal Communication of Tetrahymena

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