Studies on the Phospholipid Metabolism in Choline-Deficient Guinea Pigs

Studies on the Phospholipid Metabolism in Choline-Deficient Guinea Pigs

“…A similar discussion will here again occur as before (5). In the control group the None-preincubated slices incorpo rated more than a half amount of radioactive…”

“…Choline chloride (0.2%), L-methionine (0.2%) and an indicated amount of MME were sup plemented in several combinations to the basal diet prviously reported (4,5). Since free MME is strongly basic, it was neutralized with 6N HCl before being added to the diet as a 10% aqueous solution.…”

“…When the lipids extracted from livers of MME-fed guinea pigs (M-lipid) were added to the reaction system containing liver microsomes from control or choline-deficient animals, the incorporation of labeled methyl group from S-adenosylmethionine-14CH3 was markedly enhanced. 5. After addition of M-lipid, liver microsomes from choline-deficient guinea pigs incorporated twice as much methyl group of S-adenosylmethionine-14CH3 into phospholipids than those from control ones.…”

“…Young guinea pigs cannot grow on a choline-deficient diet (1)(2)(3)(4)(5), and the reason has been ascribed to the inability of guinea pig liver to methylate phosphatidylethanola mine to form lecithin (2,6). MME was re ported to be ineffective as a substitute for choline without the simultaneous supply of methionine (1) or betaine (2) in the diet.…”

Liver phospholipids of rats fed a choline‐deficient diet supplemented with choline or methionine

“…Phosphatidyl choline was shown to be derived through the sequential methylation of phosphatidyl ethanolamine in the liver of choline-deficient rats (1)(2)(3)(4)(5). A decrease in the total amount of phosphatidyl choline was accompanied by a reduced level of arachidonic acid in phosphatidyl ethanolamine (6).…”