Studies on the Phospholipid Metabolism in Choline-Deficient Guinea Pigs

Kobayashi, Misato

doi:10.5925/jnsv1954.16.119

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The administration of different N-methylethanolamines will influence phospholipid methylation in yeast (Waechter & Lester, 1973), intact rat liver (Kobayashi, 1970) and isolated hepatocytes (Sundler & Akesson, 1975). As observed earlier (Sundler & Akesson, 1975) N-methylethanolamine and NNdimethylethanolamine increase the incorporation of [14C]methionine into phospholipids and decrease the conversion of [3H]phosphatidylethanolamine into phosphatidylcholine.…”

Section: Discussionmentioning

confidence: 99%

Effects of analogues of ethanolamine and choline on phospholipid metabolism in rat hepatocytes

Åkesson

1977

Biochemical Journal

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1. Analogues of ethanolamine and choline were incubated with different labelled precursors of phospholipids and isolated hepatocytes and the effects on phospholipid synthesis were studied. 2. 2-Aminopropan-1-ol and 2-aminobutan-1-ol were the most efficient inhibitors of [(14)C]ethanolamine incorporation into phospholipids, whereas the incorporation of [(3)H]choline was inhibited most extensively by NN-diethylethanolamine and NN-dimethylethanolamine. 3. When the analogues were incubated with [(3)H]glycerol and hepatocytes, the appearance of (3)H in unnatural phospholipids indicated that they were incorporated, at least in part, via CDP-derivatives. The distribution of [(3)H]glycerol among molecular species of phospholipids containing 2-aminopropan-1-ol and 1-aminopropan-2-ol was the same as in phosphatidylethanolamine. In other phospholipid analogues the distribution of (3)H was more similar to that in phosphatidylcholine. 4. NN-Diethylethanolamine stimulated both the conversion of phosphatidylethanolamine into phosphatidylcholine and the incorporation of [Me-(14)C]methionine into phospholipids. Other N-alkyl- or NN-dialkyl-ethanolamines also stimulated [(14)C]methionine incorporation, but inhibited the conversion of phosphatidylethanolamine into phosphatidylcholine. This indicates that phosphatidyl-NN-diethylethanolamine is a poor methyl acceptor, in contrast with other N-alkylated phosphatidylethanolamines. 5. These results on the regulation of phospholipid metabolism in intact cells are discussed with respect to the possible control points. They also provide guidelines for future experiments on the manipulation of phospholipid polar-headgroup composition in primary cultures of hepatocytes.

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Section: Discussionmentioning

confidence: 99%

Effects of analogues of ethanolamine and choline on phospholipid metabolism in rat hepatocytes

Åkesson

1977

Biochemical Journal

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Quantitation of phosphatidyl N‐methyl and N,N‐dimethyl aminoethanol in liver and lung of N‐methylaminoethanol fed rats

Katyal

Lombardi

1974

Lipids

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A diet supplemented with N‐methylaminoethanol was fed to rats, and liver and lung phospholipids were resolved by a two dimensional thin layere chromatographic method affording the separation of phosphatidyl N‐methylaminoethanol and phosphatidyl N,N‐dimethylaminoethanol, as well as of the major phosphatides. Significant amounts of phosphatidyl N‐methylaminoethanol and phosphatidyl N,N‐dimethylaminoethanol accumulated within 24 hr in liver and lung. The fatty acid composition of liver phosphatidyl N‐methylaminoethanol and phosphatidyl N,N‐dimethylaminoethanol was determined. Stearic, arachidonic, and docosahexaenoic acids were the predominant fatty acids of these phosphatides. After injection of (Me‐14C)methionine, only phosphatidyl N,N‐dimethylaminoethanol and phosphatidylcholine became heavily labeled in both liver and lung. These findings indicate that N‐methylaminoethanol is incorporated into phosphatidyl N‐methylaminoethanol, and the latter is methylated to phosphatidyl N,N‐dimethylaminoethanol and phosphatidylcholine.

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Nutrition, Nutritional Diseases, and Nutrition Research Applications

Navia¹,

Hunt²

1976

The Biology of the Guinea Pig

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Studies on the Phospholipid Metabolism in Choline-Deficient Guinea Pigs

Cited by 3 publications

References 6 publications

Effects of analogues of ethanolamine and choline on phospholipid metabolism in rat hepatocytes

Effects of analogues of ethanolamine and choline on phospholipid metabolism in rat hepatocytes

Quantitation of phosphatidyl N‐methyl and N,N‐dimethyl aminoethanol in liver and lung of N‐methylaminoethanol fed rats

Nutrition, Nutritional Diseases, and Nutrition Research Applications

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