Studies on the Pituitary "Fettstoffwechselhormon" - IV. Comparisons Between the Lipolytic Activity of ACTH and Some Fractions of Porcine Pituitaries In Vitro

Abstract

Tbe lipolytic activity of some fractions isolated from porcine pituitary glands was studied in vitro on isolated rat fat tissue. As the check.ed fractions showed cortiootropic activity also, their lipolytic effect was oompared with that of ACfIl For the pituitary gland fraction with the highest lipolytic efTect it oould be shown that tbis lipolytic activity was not due or only to a small extent due to a oontamination with ACfH.Horm. Metab. Res. 3: 167-170 (1971) K e y -W 0 r d s: Lipolytic Hormone -Adrenocomcotropic Hormone

Studies on the Pituitary "Fettstoffwechselhormon" - V. The Further Purification of Lipolytic Active Peptides from Hog Pituitary Glands

“…The peptides P-LF I, P-LF 11, and P-LF III isolated from fraction P N by gel chromatography (Schleyer et al 1970) were also lipolytically active in vitro (Schleyer et al 1971). Examinations on disc electrophoresis at pH 9 (tris-hydrochloric acid-buffer) showed that these substances were still composed of several fractions.…”

“…The fractions eluated from CM-cellulose were lyophilized and fina\1y desalted on Sephadex G 25 medium (40 x 2,5 cm) with 0.1 N acetic acid as eluan t. The lipolytic activity of the P-LF -fractions was tested on isolated rat fat tissue according to a method described recently (Schleyer et al 1971). The corticotropie activity was measured with a radioimmunological method (Voigt, Fehm and Pfeiffer 1971).…”

“…To our knowledge the adrenocorticotropic hormone (ACTH) is supposed to be the most lipolytic active peptide hormone of the pituitary gland. However, further investigations showed that at least for one of these fractions (P-LF 11) the lipolytic activity cannot be explained only by its contents or contamination of ACTH; only 10-20% of the lipolytic activity of the fraction pol F 11 could be traced back to an irnpurity with ACTH (Schleyer et al 1971).…”