Studies on the Preservation of Leptospirae by Freeze‐drying

Otsuka, Shuzo; Manako, Kenji

doi:10.1111/j.1348-0421.1961.tb00194.x

Cited by 8 publications

(1 citation statement)

References 11 publications

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“…Otsuka and Manako (1961) have described recoveries of leptospirae while Hanson and Cannefax (1964) have reported recoveries of Treponema and Borrelia after lyophilization.…”

mentioning

confidence: 99%

RECOVERIES OF STRIGOMONAS ONCOPELTI, LEPTOSPIRAE AND REITER'S TREPONEME FROM DESICCATES AFTER STORAGE FOR FIVE YEARS

Di¹

1965

Aust J Exp Biol Med

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Summary.Desiccates of Strigomonas oncopelti, four species of leptospirae and the Reiter treponeme have yielded viable organisms after storage for five years at 4°. At room temperature only the treponeme was successfully preserved.Among the microorganisms about whieh there is only scanty information on preservation by drying arc those listed in the title of this note. These organisms were dried sonic years ago, and reports of their recoveries after 12-18 months of storage were made (Annear, 1961; Annear, 1962a; Annear, 1962b). The present note summarises the recoveries obtained after approximately five years of storage (Table 1). Several desiccates of each organism were examined. The drying methods and procedures for recovering the organisms are described in the papers cited above.

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“…Otsuka and Manako (1961) have described recoveries of leptospirae while Hanson and Cannefax (1964) have reported recoveries of Treponema and Borrelia after lyophilization.…”

mentioning

confidence: 99%

RECOVERIES OF STRIGOMONAS ONCOPELTI, LEPTOSPIRAE AND REITER'S TREPONEME FROM DESICCATES AFTER STORAGE FOR FIVE YEARS

Di¹

1965

Aust J Exp Biol Med

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A simple technique for long‐term preservation of leptospires

Samir

Wasfy

2012

J. Basic Microbiol

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The viability of six serovars of Leptospira spp. was studied after long storage at −70°C. The bacteria were either preserved in Ellinghausen–McCullough–Johnson–Harris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark‐ground microscopy over a period of 20 months at −70°C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long‐term preservation of such Leptospira.

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Long-term preservation of Leptospira spp.: challenges and prospects

Philip

Garba

Neela

2018

Appl Microbiol Biotechnol

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Preservation of leptospiral cultures is tantamount to success in leptospiral diagnostics, research, and development of preventive strategies. Each Leptospira isolate has imperative value not only in disease diagnosis but also in epidemiology, virulence, pathogenesis, and drug development studies. As the number of circulating leptospires is continuously increasing and congruent with the importance to retain their original characteristics and properties, an efficient long-term preservation is critically needed to be well-established. However, the preservation of Leptospira is currently characterized by difficulties and conflicting results mainly due to the biological nature of this organism. Hence, this review seeks to describe the efforts in developing efficient preservation methods, to discover the challenges in preserving this organism and to identify the factors that can contribute to an effective long-term preservation of Leptospira. Through the enlightenment of the previous studies, a potentially effective method has been suggested. The article also attempts to evaluate novel strategies used in other industrial and biotechnological preservation efforts and consider their potential application to the conservation of Leptospira spp.

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Studies on the Preservation of Leptospirae by Freeze‐drying

Cited by 8 publications

References 11 publications

RECOVERIES OF STRIGOMONAS ONCOPELTI, LEPTOSPIRAE AND REITER'S TREPONEME FROM DESICCATES AFTER STORAGE FOR FIVE YEARS

RECOVERIES OF STRIGOMONAS ONCOPELTI, LEPTOSPIRAE AND REITER'S TREPONEME FROM DESICCATES AFTER STORAGE FOR FIVE YEARS

A simple technique for long‐term preservation of leptospires

Long-term preservation of Leptospira spp.: challenges and prospects

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