Studies on the preservation of leptospirae by freeze-drying

Otsuka, Shuzo; Manako, Kenji

doi:10.3412/jsb.16.814

Cited by 3 publications

(3 citation statements)

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“…The effect of age and concentration of the cultures was considered as an important factor for the successful recovery of leptospires. Only 7–14 d old cultures with a count of 3 × 10 8 cells per ml were stored, based on previous reports that showed that recovery of leptospira cultures at the log‐phase of growth had higher survival rate than cultures at the stationary phase .…”

Section: Resultsmentioning

confidence: 99%

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A simple technique for long‐term preservation of leptospires

Samir

Wasfy

2012

J. Basic Microbiol

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The viability of six serovars of Leptospira spp. was studied after long storage at −70°C. The bacteria were either preserved in Ellinghausen–McCullough–Johnson–Harris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark‐ground microscopy over a period of 20 months at −70°C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long‐term preservation of such Leptospira.

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Section: Resultsmentioning

confidence: 99%

“…Up till now, a simple and feasible protocol for the prolonged conservation of leptospiral cultures that produces satisfactory results has not been established . Many protocols have reported inconsistent results for storing leptospires . The common method entails periodic subculture at 3 months intervals into Fletcher semisolid media.…”

Section: Introductionmentioning

confidence: 99%

A simple technique for long‐term preservation of leptospires

Samir

Wasfy

2012

J. Basic Microbiol

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“…In this study, failures t o recover leptospiras culturally after long-term preservation, other than those related to bacterial contamination, were attributable to sparse concentration of leptospiras at the time of freezing. Concentration of cells was also found to be a critical factor for successful recovery of leptospiras preserved by freeze-drying (6).…”

Section: Discussionmentioning

confidence: 97%