Studies on the primary structure and biological activity of a human neurophysin

Foss, Irene; Sletten, Knut; Trygstad, O

doi:10.1016/0014-5793(73)80640-4

Cited by 27 publications

(14 citation statements)

References 11 publications

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“…Exclusion chromatography of pooled human posterior pituitary extracts revealed almost all ofthe neurophysin activities to be in the fractions corresponding to the molecular size of 10,000 daltons, which is in good agreement with the molecular weight estimate of human neurophysins in previous reports (14,15). On electrophoresis, they were separated into two NSN peaks and one ESN peak.…”

Section: Discussionsupporting

confidence: 89%

Nature of the immunoreactive neurophysins in ectopic vasopressin-producing oat cell carcinomas of the lung. Demonstration of a putative common precursor to vasopressin and neurophysin.

Yamaji¹,

Ishibashi²,

Katayama³

1981

J. Clin. Invest.

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A B S T R A C T In an attempt to delineate the nature of the immunoreactive neurophysins in oat cell carcinomas of the lung with ectopic vasopressin production, tumor neurophysins were characterized by gel filtration and by electrophoresis. In all ofthe five tumor tissues, activities of both vasopressin and nicotinestimulated neurophysin (NSN) determined by radioimmunoassay were demonstrated. A small amount of oxytocin as well as estrogen-stimulated neurophysin was detected in three of the tissues. When tissue extract was subjected to Sephadex G-50 gel filtration in 0.2 N acetic acid, the major portion of immunoreactive NSN emerged in the fractions corresponding to the molecular size of 10,000. The migration pattern of NSN in these fractions on electrophoresis was qualitatively the same as that of NSN extracted from human posterior pituitary glands. In addition to this major neurophysin, immunoreactive NSN with the molecular size of 20,000 was consistently demonstrated in three tumor extracts. This high molecular weight form of neurophysin represented 6.5-8.7% of total NSN immunoactivities in each tumor extract and its elution profile was not changed when analyzed under denaturating conditions in 6 M guanidine hydrochloride. On electrophoresis, it migrated near the gamma globulin region; however, the peak was broad suggesting that it consists of more than two different molecular populations. A substantial portion of the high molecular weight NSN appears to be a glycoprotein judging from its binding to concanavalin A. When the high molecular weight form of neurophysin This study was presented in part at the Sixth Intemational

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Section: Discussionsupporting

confidence: 89%

Nature of the immunoreactive neurophysins in ectopic vasopressin-producing oat cell carcinomas of the lung. Demonstration of a putative common precursor to vasopressin and neurophysin.

Yamaji¹,

Ishibashi²,

Katayama³

1981

J. Clin. Invest.

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“…However, the results obtained by automated Edman degradation on the unfractionated neurophysins reveal that a second neurophysin having the same N-terminal sequence as that found for neurophysin I [3,6] is also present. As suggested [7], neurophysin I probably belongs to the VLDV neurophysin lineage, due to the nature of residues 6, 7 and 9.…”

Section: Unfractionated Neurophysinsmentioning

confidence: 91%

“…It is clear that this fraction is a mixture of two neurophysins which have identical residues in positions 1,4,5,8, 10 and 11 but different residues in positions 2,3,6,7 and 9. The N-terminal sequence of human neurophysin I is known [3,6];because this protein has alanine, proline, aspartic acid, valine and lysine, respectively, in positions 2, 3,6,7 and 9, by difference the N-terminal sequence Ala-Met-Ser-Asp-Leu-Glu-Leu-ArgGln-Cys-Leu can be assigned to the second neurophysin. This agrees with the sequence deduced from peptides Tl and T2.…”

Section: Unfractionated Neurophysinsmentioning

confidence: 99%

“…Two components have been purified [2] designated neurophysin I and II, according to their electrophoretie mobilities. The N-terminal sequence of neurophysin 1 has been established [3,6] but there is no structural information about neurophysin Il.…”

Section: Introductionmentioning

confidence: 99%

“…It seems therefore that each mammalian species has one neurophysin of each type. From the N-terminal sequence of human neurophysin 1 [3,6] we have deduced [7] that this neurophysin should belong to the VLDV neurophysin family. If so a representative of the second type should exist along with the VLDV neurophysin.…”

Section: Introductionmentioning

confidence: 99%

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