Studies on the uptake of tomato lectin nanoparticles in everted gut sacs

Carreño-Gómez, Begoña; Woodley, John; Florence, Alexander T.

doi:10.1016/s0378-5173(99)00050-2

Cited by 59 publications

(12 citation statements)

References 5 publications

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“…Lectins, carbohydrate-binding proteins capable of binding to glycosylated proteins and cell membrane components to provide muco- and cytoadhesion [14], have been functionalized onto drug delivery systems to promote adhesion to the lining of the GI tract [6, 51–53, 59–61]. PMMA microdevices modified with tomato lectin (Figure 3 B), which binds selectively to the epithelium of the small intestine [62], demonstrated 92 ± 4% retention in an in vitro Caco-2 adhesion assay, whereas devices lacking modification showed 29 ± 9% retention [51]. In vivo, lectin-conjugated PMMA microdevices showed 41% retention in the proximal small intestine of mice two hours following oral administration as opposed to 27% for bare devices [53].…”

Section: Strategies To Increase Micro/nanofabricated Oral Drug Delmentioning

confidence: 99%

Micro/nanofabricated platforms for oral drug delivery

Fox

Kim

et al. 2015

Journal of Controlled Release

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The oral route of drug administration is most preferred due to its ease of use, low cost, and high patient compliance. However, the oral uptake of many small molecule drugs and biotherapeutics is limited by various physiological barriers, and, as a result, drugs suffer from issues with low solubility, low permeability, and degradation following oral administration. The flexibility of micro- and nanofabrication techniques has been used to create drug delivery platforms designed to address these barriers to oral drug uptake. Specifically, micro/nanofabricated devices have been designed with planar, asymmetric geometries to promote device adhesion and unidirectional drug release toward epithelial tissue, thereby prolonging drug exposure and increasing drug permeation. Furthermore, surface functionalization, nanotopography, responsive drug release, motion-based responses, and permeation enhancers have been incorporated into such platforms to further enhance drug uptake. This review will outline the application of micro/nanotechnology to specifically address the physiological barriers to oral drug delivery and highlight technologies that may be incorporated into these oral drug delivery systems to further enhance drug uptake.

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Section: Strategies To Increase Micro/nanofabricated Oral Drug Delmentioning

confidence: 99%

Micro/nanofabricated platforms for oral drug delivery

Fox

Kim

et al. 2015

Journal of Controlled Release

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“…In cattle, CD205 is expressed at high levels on various subpopulations of DCs, but is also present at lower levels on B cells, T cells and macrophages 44 . Gliddon and coworkers 45 found that large mononuclear cells in afferent lymph draining from the skin of cattle expressed high levels of CD205 and also expressed high levels of MHC II. Interestingly, antigens presented by CD205 + DCs in the absence of inflammatory stimuli have been found to induce tolerance 46 , 47 .…”

Section: Discussionmentioning

confidence: 99%

Phenotypic characterization of cells participating in transport of prion protein aggregates across the intestinal mucosa of sheep

Åkesson

Press

Tranulis

et al. 2012

Prion

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The oral route is considered to be the main entry site of several transmissible spongiform encephalopathies or prion diseases of animals and man. Following natural and experimental oral exposure to scrapie, sheep first accumulate disease associated prion protein (PrP d ) in Peyer’s patch (PP) lymphoid follicles. In this study, recombinant ovine prion protein (rPrP) was inoculated into gut loops of young lambs and the transportation across the intestinal wall studied. In particular, the immunohistochemical phenotypes of cells bearing the inoculated prion protein were investigated. The rPrP was shown to be transported across the villi of the gut, into the lacteals and submucosal lymphatics, mimicking the transport route of PrP d from scrapie brain inoculum observed in a previous intestinal loop experiment. The cells bearing the inoculated rPrP were mainly mononuclear cells, and multicolor immunofluorescence procedures were used to show that the rPrP bearing cells were professional antigen presenting cells expressing Major histocompatibility complex II (MHCII). In addition, the rPrP bearing cells labeled with CD205, CD11b and the macrophage marker CD68, and not with the dendritic cell markers CD11c and CD209. Others have reported that cells expressing CD205 and CD11b in the absence of CD11c have been shown to induce T cell tolerance or regulatory T cells. Based on this association, it was speculated that the rPrP and by extension PrP d and scrapie infective material may exploit the physiological process of macromolecular uptake across the gut, and that this route of entry may have implications for immune surveillance.

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“…At pHs greater that 7.4, the glycoprotein immobilization and/or deposition tended to decrease sharply. Carreno-Gomez et al reported that tomato lectin (TL) coupling, on carboxylated nanoparticles, was 3-4 mg of TL/mg of microspheres using carbodiimide to achieve glycoprotein binding [22].…”

Section: Ph Effect On Con a Immobilizationmentioning

confidence: 99%

Surface Modification and Covalent Coupling of Concanavalin A onto Poly(EGDMA/HEMA) Microbeads for Cell Affinity Applications

Ayhan

2003

Journal of Bioactive and Compatible Polymers

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The surfaces of nonporous and porous Poly(EGDMA/HEMA) microbeads were modified by the reaction of sodium periodate, hexamethylenediamine and glutaraldehyde. Covalent immobilization of Concanavalin A (Con A) was optimized for maximum loading at various Con A concentrations, pH, temperatures, and ionic strengths. The optimal Con A immobilized concentration was determined as 0.37 and 0.75 mg/g microbeads for the nonporous and porous microbeads, respectively. Covalent coupling was achieved at 37 C, with 0.5 mg/mL of Con A at pH 7.4 and ionic strength of 0.01. The specific activity of Con A immobilized microbeads to carbohydrate structure was tested for affinity for myeloma HeLa cells. The data indicates that Con A may play a role in myeloma cell adhesion.

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Studies on the uptake of tomato lectin nanoparticles in everted gut sacs

Cited by 59 publications

References 5 publications

Micro/nanofabricated platforms for oral drug delivery

Micro/nanofabricated platforms for oral drug delivery

Phenotypic characterization of cells participating in transport of prion protein aggregates across the intestinal mucosa of sheep

Surface Modification and Covalent Coupling of Concanavalin A onto Poly(EGDMA/HEMA) Microbeads for Cell Affinity Applications

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