Studies on the Use of Prostaglandin F2α and Gonadotropin-Releasing Hormone Analogs for Timed Artificial Insemination in Jennies

Fanelli, Diana; Tesi, Matteo; Rota, Alessandra; Beltramo, Massimiliano; Camillo, Francesco; Panzani, Duccio

doi:10.1016/j.jevs.2018.12.001

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…If the jenny does not have a CL mature enough to respond to the first PGF2a injection, in 16-17 days she should have a CL that will be responsive to the second PGF2a, thus allowing her to return to estrus (Figure 15, Table 8). Further modification of this protocol would be to administer GnRH seven days after the first PGF2a to ensure ovulation, and then to administer a second PGF2a injection 7 days later to induce luteolysis 92 (Figure 15, Table 8).…”

Section: Hormonal Manipulation Of the Estrous Cyclementioning

confidence: 99%

“…Random administration of GnRH followed by a dose of PGF2a, presumes that the jenny would have a follicle in the ovaries able to respond to the GnRH, regardless, if there is a CL present, and then the injection of PGF2a 7 days later, would induce luteolysis in the jenny if she ovulated after GnRH administration or if she already had a CL at the onset of the protocol 92 . GnRH (day 0) +PGF2a (day 8) 92 5/9 (55%) 3/5 (60%) *Denotes daily reproductive ultrasound after the last PGF2a; ** Denotes daily reproductive ultrasound starting two days after intravaginal progesterone-releasing device removal; ***Denotes administration of buserelin acetate (0.4 mg/jenny, SC), seven days the second PGF2a injection and then AI with fresh extended semen.…”

Section: Hormonal Manipulation Of the Estrous Cyclementioning

confidence: 99%

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Key Aspects of Donkey and Mule Reproduction

Canisso

Panzani

Miró

et al. 2019

Veterinary Clinics of North America: Equine Practice

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Section: Hormonal Manipulation Of the Estrous Cyclementioning

confidence: 99%

Section: Hormonal Manipulation Of the Estrous Cyclementioning

confidence: 99%

Key Aspects of Donkey and Mule Reproduction

Canisso

Panzani

Miró

et al. 2019

Veterinary Clinics of North America: Equine Practice

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“…Moreover, in the last 20 years, with the discovery of the nutraceutical capabilities of donkey milk and more and more after the massive use of donkeys to produce donkey hide gelatin, the interest in donkey as a food-producing animal increased (Panzani et al, 2020). In this new productive view, the donkey assisted reproductive techniques started to develop with several studies focusing on oestrous and ovulation synchronization (Fanelli et al, 2018(Fanelli et al, , 2019Liu et al, 2020;Yang et al, 2018;Zhou et al, 2018) to optimize the reproductive management of the donkey farms with timed artificial insemination and embryo transfer. To induce ovulation in the jenny hCG, GnRH analogues (Camillo et al, 2014;Carluccio et al, 2007;Liu et al, 2020) and Kisspeptin (Fanelli et al, 2022) have been used.…”

Section: Discussionmentioning

confidence: 99%

GnRH analogue triptorelin acetate induces ovulation in jennies during oestrus

Fanelli,

Moroni,

Berti

et al. 2024

Reprod Domestic Animals

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Most of the northern hemisphere donkey breeds are faced with the risk of extinction, thus donkey reproduction is considered an emerging branch of theriogenology, and the management of artificial insemination and induction of ovulation is a crucial point in setting up preservation protocols. For four consecutive cycles, six jennies' ovarian activity was routinely monitored; an ultrasound examination was performed daily from the evidence of a follicle diameter ≥27 mm until ovulation. Upon reaching a follicular diameter ≥32 ± 2 mm (Hour 0), oestrous jennies were treated alternatively with 0.1 mg triptorelin acetate, sc, (TRIP), 0.4 mg/sc of buserelin acetate (BUS) or saline, sc, (CTRL) and examined ultrasonographically at Hours 14, 24, 38, 42, 48, 62 and every 24 h until ovulation. Induction of ovulation was considered successful if ovulation occurred from 24 to 48 h after treatment. 11/12 cycles resulted in ovulation for TRIP and 12/12 for BUS and CTRL groups, respectively. Mean ± SD ovulation time after treatment was 37.3 ± 8.3, 47.1 ± 21.0 and 66.8 ± 25.9 h for BUS, TRIP and CTRL, respectively (p = .0032). Ovulation rates between h24 and h48 were 10/12 (83.3%) for both TRIP/BUS and 2/12 (16.7%) for CTRL, respectively (p = .003). Buserelin and triptorelin‐treated jennies had a 25 times higher probability to ovulate between Hours 24 and 48 than controls (p = .003), while there were no jenny and cycle effects on the ovulatory rate. The results of this study show how triptorelin successfully induced ovulation in jennies, like other GnRH analogues previously evaluated.

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Section: Introductionmentioning

confidence: 99%

“…Reproductive management on donkey farms, especially under intense rearing conditions on large farms, is challenging. One reason for this is that knowledge of ovulation induction in jennies is limited, and pregnancy rates using fresh or frozen semen in this species are unsatisfactory 1,2 and despite common belief, the direct transfer of knowledge and procedures from horses to donkeys often yields poor results. 3 In order to optimise reproductive management in mares and jennies, the goal of ovulation induction should be focused not only on hastening, but also on ensuring the occurrence of ovulation at a predictable time.…”

Section: Introductionmentioning

confidence: 99%

Practical protocols for timed artificial insemination of jennies using cooled or frozen donkey semen

Yang

Liu³

et al. 2021

Equine Veterinary Journal

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Background: With the expansion of the donkey industry, timed artificial insemination (TAI) is becoming increasingly important in the reproductive management of jennies, however, TAI has not been widely investigated in donkeys.Objectives: To develop efficient TAI protocols for cooled or frozen semen in jennies, based around ovulation induction with a GnRH analogue.Study design: Experimental exploratory study. Methods and Results:In experiment 1, the effects of different GnRH analogue (deslorelin) doses, follicle diameter (FD) at induction, repeated use of a GnRH analogue, and the influence of season on induction efficiency, as well as distribution of ovulations over time after induction were investigated. Induction efficiency was sufficient with 2.2 mg deslorelin (≥90% ovulation within 48 hours of treatment). Ovulation rate between 24 and 48 hours was highest when the FD at treatment was 31-35 mm, as compared to 25-30 mm or 36-40 mm. Repeated use of deslorelin or treatment during different seasons had no effect on induction efficiency. About 70% of ovulations occurred between 32 and 48 hours, and highest incidence of ovulation was at 36-38 hours after induction. In experiment 2, TAI using cooled semen (1 × 10 9 motile sperm in a 10 mL volume) was performed once at 8 hours after induction (n = 59).Pregnancy rate after TAI with cooled semen was 49.2% (29/59). In experiment 3, jennies were inseminated twice with 10 (n = 23), 5 (n = 31), 3 (n = 32), 2 (n = 82) and 1 (n = 66) straws (more than 50 × 10 6 motile spermatozoa in each 0.5 mL straw) of frozen semen at 34 and 42 hours after induction. The pregnancy rates were 30.4%, 35.5%, 34.4%, 29.3% and 28.8%, respectively (P > 0.05). Main limitations:In the frozen semen trial, 22.5% (68/302) jennies were excluded after failure to ovulate during the appropriate time interval. In addition, there were no control groups for the AI trials. Conclusion:When FD reaches 31-35 mm, a donkey jenny can be inseminated once using cooled semen at 8 hours or twice using frozen semen at 34 and 42 hours after | 1219 YANG et Al.

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Studies on the Use of Prostaglandin F2α and Gonadotropin-Releasing Hormone Analogs for Timed Artificial Insemination in Jennies

Cited by 14 publications

References 42 publications

Key Aspects of Donkey and Mule Reproduction

Key Aspects of Donkey and Mule Reproduction

GnRH analogue triptorelin acetate induces ovulation in jennies during oestrus

Practical protocols for timed artificial insemination of jennies using cooled or frozen donkey semen

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