Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

Abstract

Background The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius), respectively.Results The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level.Conclosion Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species.

Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay

“…In 2013, Zhang et al developed a LAMP assay using the gene encoding pyolysin, the plo gene, for a specific identification of T. pyogenes (Zhang et al 2013). Furthermore, a pla LAMP assay was used for identifying A. pluranimalium (Abdulmawjood et al 2015), and a cpn60 LAMP assay for identifying T. pyogenes from different animal origins (Abdulmawjood et al 2016;Ahmed et al 2020;Alssahen et al 2020).…”

Phenotypic and genotypic approach to characterize a Trueperella pecoris strain isolated from necrotic vestibulitis of a camel (Camelus dromedarius)

“…Cultural and biochemical characterization as well as CAMP-like hemolytic reactions of the Trueperella isolate were investigated as described previously for other bacteria of the genus Trueperella and Arcanobacterium (Hijazin et al 2011;Eisenberg et al 2012;Ahmed et al 2020;Alssahen et al 2020). Detailed biochemical properties were determined in accordance with the manufacturer's instructions using the API-Coryne test system (BioM erieux Deutschland GmbH, N€ urtingen, Germany).…”

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

“…In Japanese race horses and multidrug resistance profiles [85] Enterobacteria Fecal samples from turkeys, widespread antibiotic resistance found [86] Trueperella pyogenes Samples include vaginal discharge of an okapi (Okapia johnstoni) and the kidney of a female royal python [87] Escherichia coli, Salmonella, and Acinetobacter Study of backyard poultry flocks in the United States [88]…”

“…Ahmed et al [87] used MALDI-TOF for identifying T. pyogenes isolates recovered from a vaginal discharge of an okapi (Okapia johnstoni) and the kidney of a female royal python. Shah et al [88] reported on an emerging One Health concern -the growing popularity of backyard poultry flocks in the United States and corresponding increased risk of human-animal contact and transmission of pathogens.…”